中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
12期
2127-2130
,共4页
吴国强%李开宗%窦科峰%药立波%刘新平
吳國彊%李開宗%竇科峰%藥立波%劉新平
오국강%리개종%두과봉%약립파%류신평
癌,肝细胞%NDRG2%转染
癌,肝細胞%NDRG2%轉染
암,간세포%NDRG2%전염
Carcinoma,hepatocellular%NDRG2%Transfection
目的 观察NDRG2基因(N-myc downstream regulated gene 2)对肝癌细胞株HepG2生物学行为的影响.方法 采用脂质体法将正义及反义NDRG2基因稳定转染HepG2及QZG细胞,采用逆转录-聚合酶链反应(RT-PCR)、免疫组织化学及Western blot法进行鉴定；比较观察其生物学特性的变化.结果 NDRG2基因在NDRG2-HepG2细胞中得到了稳定表达；NDRG2-HepG2与HepG2细胞在细胞计数(1、2、3、4、5、6、7d分别为3 × 105/6×105、4×105/12×105、5×105/13×105、6.0×105/13.5×105、6.5×105/15.0×105、6.8×105/16.5×105、7×105/17×105)、细胞集落形成(32.4±4.7/56.5±5.6)及成瘤重量(0.0462 g/0.2120 g)差异有统计学意义(P＜0.05)；而Anti-QZG与QZG细胞的差异无统计学意义(P＞0.05).结论 NDRG2基因可抑制肝癌细胞株HepG2细胞生长增殖.
目的 觀察NDRG2基因(N-myc downstream regulated gene 2)對肝癌細胞株HepG2生物學行為的影響.方法 採用脂質體法將正義及反義NDRG2基因穩定轉染HepG2及QZG細胞,採用逆轉錄-聚閤酶鏈反應(RT-PCR)、免疫組織化學及Western blot法進行鑒定；比較觀察其生物學特性的變化.結果 NDRG2基因在NDRG2-HepG2細胞中得到瞭穩定錶達；NDRG2-HepG2與HepG2細胞在細胞計數(1、2、3、4、5、6、7d分彆為3 × 105/6×105、4×105/12×105、5×105/13×105、6.0×105/13.5×105、6.5×105/15.0×105、6.8×105/16.5×105、7×105/17×105)、細胞集落形成(32.4±4.7/56.5±5.6)及成瘤重量(0.0462 g/0.2120 g)差異有統計學意義(P＜0.05)；而Anti-QZG與QZG細胞的差異無統計學意義(P＞0.05).結論 NDRG2基因可抑製肝癌細胞株HepG2細胞生長增殖.
목적 관찰NDRG2기인(N-myc downstream regulated gene 2)대간암세포주HepG2생물학행위적영향.방법 채용지질체법장정의급반의NDRG2기인은정전염HepG2급QZG세포,채용역전록-취합매련반응(RT-PCR)、면역조직화학급Western blot법진행감정；비교관찰기생물학특성적변화.결과 NDRG2기인재NDRG2-HepG2세포중득도료은정표체；NDRG2-HepG2여HepG2세포재세포계수(1、2、3、4、5、6、7d분별위3 × 105/6×105、4×105/12×105、5×105/13×105、6.0×105/13.5×105、6.5×105/15.0×105、6.8×105/16.5×105、7×105/17×105)、세포집락형성(32.4±4.7/56.5±5.6)급성류중량(0.0462 g/0.2120 g)차이유통계학의의(P＜0.05)；이Anti-QZG여QZG세포적차이무통계학의의(P＞0.05).결론 NDRG2기인가억제간암세포주HepG2세포생장증식.
Objective To investigate the biological effects of the NDRG2 gene on HepG2 cells.Methods The sense and anti-sense NDRG2 full length cDNA were transfected into HepG2 and QZG cell lines by the vectors of pIRES2-EGFP-NDRG2 and pIRES2-EGFP-Anti-NDRG2 respectively and the transfectants were obtained by clone selection.The expression levels of NDRG2 gene were detected by reverse transcription-polymerase chain reaction (RT-PCR),immunochemistry and Western blotting.Effects of NDRG2 and Anti-NDRG2 transfection on the biological characteristics of HepG2 and QZG cells were analyzed,including proliferation rate,colony-forming efficiency (CFE) and tumor formation.Results The NDRG2-HepG2 cell line was obtained after stable gene transfection.There was significant difference between NDRG2-HepG2 and HepG2 cells (P ＜0.05) in the cell count (1,2,3,4,5,6,7 days after passage culture:3 × 105 vs.6×105,4×105 vs.12×105,5×105 vs.13×105,6.0×105 vs.13.5×105,6.5×105 vs.15.0×105,6.8 × 105 vs.16.5 × 105,7 × 105 vs.17 × 105 respectively).There was no significant difference in the CFE (32.4 ±4.7 vs.56.5 ±5.6) and the tumor formation (0.0462 g vs.0.2120 g) between Anti-QZG and QZG cells (P ＞0.05 ).Conclusion NDRG2 gene can arrest the growth of HepG2 cells.
