中华消化内镜杂志
中華消化內鏡雜誌
중화소화내경잡지
CHINESE JOURNAL OF DIGESTIVE ENDOSCOPY
2010年
3期
145-148
,共4页
幽门螺杆菌%多态性,单核苷酸%抗生素耐受性%细菌%突变分析%DNA
幽門螺桿菌%多態性,單覈苷痠%抗生素耐受性%細菌%突變分析%DNA
유문라간균%다태성,단핵감산%항생소내수성%세균%돌변분석%DNA
Helicobacter pylori%Polymorphism,single nucleotide%Antibiotic resistance,bacterial%Mutational analysis,DNA
目的 研究幽门螺杆菌(Helicobacter priori,Hp)对克拉霉素耐药情况及与23S rRNA基因点突变的关系.方法 因上消化道症状进行胃镜检查的189例患者获得胃活检组织,微需氧培养得到Hp,提取11例敏感菌和和19例耐药菌的DNA,对23S rRNA基因进行PCR扩增,再对敏感菌和耐药菌的23S rRNA基因进行全基因测序对比和生物信息学分析.结果 Hp菌株对克拉霉素的耐药率是29.2%;19个对克拉霉素耐药的Hp菌株中17株出现23S rRNA基因突变,各种突变的比例分别为A→G 36.8%、G→A 21.5%、C→T 15.8%、A→C 10.5%和T→C 5.3%.11例敏感株及2例耐药株均未发现23S rRNA基因突变.结论 克拉霉素耐药的Hp菌株比较常见,23S rRNA基因的多个不同位点突变均与Hp对克拉霉素耐药有关,而A→G和G→A突变是主要的形式.
目的 研究幽門螺桿菌(Helicobacter priori,Hp)對剋拉黴素耐藥情況及與23S rRNA基因點突變的關繫.方法 因上消化道癥狀進行胃鏡檢查的189例患者穫得胃活檢組織,微需氧培養得到Hp,提取11例敏感菌和和19例耐藥菌的DNA,對23S rRNA基因進行PCR擴增,再對敏感菌和耐藥菌的23S rRNA基因進行全基因測序對比和生物信息學分析.結果 Hp菌株對剋拉黴素的耐藥率是29.2%;19箇對剋拉黴素耐藥的Hp菌株中17株齣現23S rRNA基因突變,各種突變的比例分彆為A→G 36.8%、G→A 21.5%、C→T 15.8%、A→C 10.5%和T→C 5.3%.11例敏感株及2例耐藥株均未髮現23S rRNA基因突變.結論 剋拉黴素耐藥的Hp菌株比較常見,23S rRNA基因的多箇不同位點突變均與Hp對剋拉黴素耐藥有關,而A→G和G→A突變是主要的形式.
목적 연구유문라간균(Helicobacter priori,Hp)대극랍매소내약정황급여23S rRNA기인점돌변적관계.방법 인상소화도증상진행위경검사적189례환자획득위활검조직,미수양배양득도Hp,제취11례민감균화화19례내약균적DNA,대23S rRNA기인진행PCR확증,재대민감균화내약균적23S rRNA기인진행전기인측서대비화생물신식학분석.결과 Hp균주대극랍매소적내약솔시29.2%;19개대극랍매소내약적Hp균주중17주출현23S rRNA기인돌변,각충돌변적비례분별위A→G 36.8%、G→A 21.5%、C→T 15.8%、A→C 10.5%화T→C 5.3%.11례민감주급2례내약주균미발현23S rRNA기인돌변.결론 극랍매소내약적Hp균주비교상견,23S rRNA기인적다개불동위점돌변균여Hp대극랍매소내약유관,이A→G화G→A돌변시주요적형식.
Objective To investigate the resistance rate of Helicobacter pylori (Hp) to clarithromycin and its correlation with point mutations in 23S rRNA gene. Methods Hp was cultured from gastric biopsy specimen obtained from 189 patients undergoing upper gastrointestinal endoscopy. The DNA of 11 clarithromycin sensitive Hp and 19 clarithromycin resistant Hp was extracted, and 23S rRNA was amplified and sequenced. Results The rate of clarithromycin resistance in cultured Hp was 29. 2%. Point mutations in 23S rRNA gene were found in 17 clarithromycin resistant Hp strains. The proportion of A to G mutation was 36.8%, G to A of 21.5%, C to T of 15.8%, A to C of 10.5% and T to C of 5.3%. No point mutation in 23S rRNA was detected in other 2 clarithromycin resistant and 11 sensitive Hp strains. Conclusion The resistance to clarithromycin is common in Hp, and point mutations in 23S rRNA gene of Hp are frequent in clarithromycin resistant strains, with most prevalent mutations of A to G and next G to A.
