基质金属蛋白酶及其抑制因子在非黑色素性皮肤癌中的表达及临床意义
기질금속단백매급기억제인자재비흑색소성피부암중적표체급림상의의
Expression and significance of matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase in non-melanoma skin cancer
  • 万方数据
    中华肿瘤杂志 중화종류잡지
    CHINESE JOURNAL OF ONCOLOGY
    2012年 5期 369-373 ,共5页

    付晓瑞%张展%陈长英%张蕾%王留兴%汪变红%柳喜洋%张明智

    부효서%장전%진장영%장뢰%왕류흥%왕변홍%류희양%장명지

    皮肤肿瘤%基质金属蛋白酶%基质金属蛋白酶组织抑制物 皮膚腫瘤%基質金屬蛋白酶%基質金屬蛋白酶組織抑製物
    피부종류%기질금속단백매%기질금속단백매조직억제물
    Skin neoplasms%Matrix metalloproteinase%Tissue inhibitor of matrix metalloproteinase
    目的 探讨基质金属蛋白酶2 (MMP-2)和MMP-9及基质金属蛋白酶组织抑制物1(TIMP-1)和TIMP-2在非黑色素性皮肤癌(NMSC)中的表达及其临床意义.方法 病理证实的NMSC患者组织标本68例,其中鳞状细胞癌(SCC)36例,基底细胞癌(BCC)32例,取肿瘤中心组织和边缘癌组织；同时,以30例正常皮肤组织为对照组.应用免疫组织化学法和逆转录聚合酶链反应( RT-PCR)法,检测各组中MMP-2、MMP-9、TIMP-1和TIMP-2的表达情况.结果 对照组中MMP-2和MMP-9的阳性率分别为30.0％和36.7％,MMP-2和MMP-9的表达水平分别为57.216±12.785和59.318±13.262,均显著低于SCC组和BCC组的中心及边缘癌组织(P＜0.01)；对照组中TIMP-1和TIMP-2的阳性率分别为96.7％和100％,TIMP-1和TIMP-2的表达水平分别为121.738±25.516和122.612±25.964,均显著高于SCC组和BCC组的中心及边缘癌组织(P＜0.01).SCC组中心及边缘癌组织中MMP-2和MMP-9的表达水平显著高于BCC组中心及边缘癌组织,而TIMP-1和TIMP-2表达水平显著低于BCC组中心及边缘癌组织(P＜0.01).SCC组和BCC组边缘癌组织中,MMP-2的表达水平分别为118.438±28.517和101.672±19.137,MMP-9的表达水平分别为115.816±26.416和99.731±19.058; SCC组和BCC组中心癌组织中,MMP-2的表达水平分别为97.197±18.359和87.825±17.351,MMP-9的表达水平分别为95.732±18.157和85.182±16.973,SCC组和BCC组边缘癌组织中MMP-2和MMP-9的表达水平明显高于中心癌组织(P＜0.01).SCC组和BCC组边缘癌组织中,TIMP-1的表达水平分别为82.872±16.285和91.653±17.197,TIMP-2的表达水平分别为83.157±16.174和91.926±17.283；SCC组和BCC组中心癌组织中,TIMP-1的表达水平分别为93.561±17.037和103.356±19.738,TIMP-2的表达水平分别为94.019±17.046和104.191±19.887,SCC组和BCC组边缘癌组织中TIMP-1和TIMP-2的表达水平明显低于中心癌组织(P＜0.01).结论 MMP-2、MMP-9及其抑制因子TIMP-1、TIMP-2与NMSC的发生、发展密切相关,可能在NMSC的侵袭与转移中有重要作用.
    목적 탐토기질금속단백매2 (MMP-2)화MMP-9급기질금속단백매조직억제물1(TIMP-1)화TIMP-2재비흑색소성피부암(NMSC)중적표체급기림상의의.방법 병리증실적NMSC환자조직표본68례,기중린상세포암(SCC)36례,기저세포암(BCC)32례,취종류중심조직화변연암조직；동시,이30례정상피부조직위대조조.응용면역조직화학법화역전록취합매련반응( RT-PCR)법,검측각조중MMP-2、MMP-9、TIMP-1화TIMP-2적표체정황.결과 대조조중MMP-2화MMP-9적양성솔분별위30.0％화36.7％,MMP-2화MMP-9적표체수평분별위57.216±12.785화59.318±13.262,균현저저우SCC조화BCC조적중심급변연암조직(P＜0.01)；대조조중TIMP-1화TIMP-2적양성솔분별위96.7％화100％,TIMP-1화TIMP-2적표체수평분별위121.738±25.516화122.612±25.964,균현저고우SCC조화BCC조적중심급변연암조직(P＜0.01).SCC조중심급변연암조직중MMP-2화MMP-9적표체수평현저고우BCC조중심급변연암조직,이TIMP-1화TIMP-2표체수평현저저우BCC조중심급변연암조직(P＜0.01).SCC조화BCC조변연암조직중,MMP-2적표체수평분별위118.438±28.517화101.672±19.137,MMP-9적표체수평분별위115.816±26.416화99.731±19.058; SCC조화BCC조중심암조직중,MMP-2적표체수평분별위97.197±18.359화87.825±17.351,MMP-9적표체수평분별위95.732±18.157화85.182±16.973,SCC조화BCC조변연암조직중MMP-2화MMP-9적표체수평명현고우중심암조직(P＜0.01).SCC조화BCC조변연암조직중,TIMP-1적표체수평분별위82.872±16.285화91.653±17.197,TIMP-2적표체수평분별위83.157±16.174화91.926±17.283；SCC조화BCC조중심암조직중,TIMP-1적표체수평분별위93.561±17.037화103.356±19.738,TIMP-2적표체수평분별위94.019±17.046화104.191±19.887,SCC조화BCC조변연암조직중TIMP-1화TIMP-2적표체수평명현저우중심암조직(P＜0.01).결론 MMP-2、MMP-9급기억제인자TIMP-1、TIMP-2여NMSC적발생、발전밀절상관,가능재NMSC적침습여전이중유중요작용.
    Objective To investigate the expression and significance of matrix metalloproteinases (MMP-2,MMP-9) and tissue inhibitor of matrix metalloproteianse (TIMP-2,TIMP-1 ) in non-melanoma skin cancer (NMSC).Methods Thirty six patients with squamous cell carcinoma (SCC) and 32 patients with basal cell carcinoma (BCC),confirmed by pathology,were selected,and 30 cases of normal skin were selected as control.The expression of MMP-2,MMP-9,TIMP-1 and TIMP-2 in all samples were examined by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).The expression rate,expression intensity and expression level of each factor were recorded.The results were compared between the groups.Results The expression rates of MMP-2 and MMP-9 in the control group were 30.0％ and 36.7％,the expression levels of MMP-2 and MMP-9 in the control group were 57.216 ± 12.785 and 59.318 ±13.262,all significantly lower than those in the tumor edge and center of the SCC and BCC groups (P ＜0.01 ).The expression rates of TIMP-1 and TIMP-2 in the control group were 96.7％ and 100％,their expression levels were 121.738 ± 25.516 and 122.612 ± 25.964,all significantly higher than those in the SCC and BCC groups ( P ＜0.01 ).The expression levels of MMP-2 and MMP-9 in the tumor center and edge of SCC group were significantly higher than those in the corresponding parts of the BCC group,while the expression levels of TIMP-1 and TIMP-2 were significantly lower than those in the BCC group (P ＜0.01 ).The expression levels of MMP-2 and MMP-9 in the tumor edge of the SCC and BCC groups were significantly higher titan those in the tumor centers (P ＜0.01 ),while the expression levels of TIMP-1and TIMP-2 were significantly lower than those in the tumor centers ( P ＜0.01 ).Conclusion MMP-2,MMP-9 and TIMP-2,TIMP-1 may play an important role in the development,progression,invasion and metastasis of nonmelanoma skin cancer.
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