CAJ | 학술논문

潜育性水稻田广泛分布于中国、斯里兰卡、印度、印度尼西亚、塞拉里昂、利比亚、尼日利亚、哥伦比亚和菲律宾等国,其中我国南方稻区就有近700万公顷低产潜育性水稻田.该类水稻田还原性强,矿质营养失调,尤以Fe2+过量积累,对水稻生长发育产生不良的逆境胁迫作用.培育抗亚铁毒的水稻品种是简便、经济有效地提高稻谷产量的重要途径之一.该文利用由粳稻品种Asominori与籼稻品种IR24杂交衍生的Asominori染色体片段置换系(Chromosome Segment Substitution Lines,CSSLs)群体为材料,检测与抗亚铁毒胁迫有关性状QTL.共检测到与抗亚铁毒胁迫有关性状QTL14个,各QTL的LOD值为2.72～6.63.其中检测到与抗亚铁毒胁迫直接有关的性状叶片棕色斑点指数QTL 3个,分别位于第3、9、11染色体C515～XNpb279、R2638～C1263和G1465～C950之间,对应的贡献率分别为16.45%、11.16%和28.02%;与其他已发表的定位结果比较发现,位于第三染色体C515～XNpb279间控制叶片棕色斑点指数的QTL与水稻功能图谱上控制叶绿素含量的QTL的位置一致;表明在亚铁毒胁迫条件下,水稻在其叶片表面出现棕色斑点,叶片衰老,产生一些叶绿素降解物或衍生物,以提高叶片细胞对亚铁等重金属毒害的耐受力.另外,在第11染色体G1465～C950之间检测到了控制叶片棕色斑点指数、茎干重和根干重QTL 1个,为主效QTL.在第6染色体XNpb386～XNpb342之间检测到控制茎干重、株高、根长和根干重QTL 1个,是否与水稻抗亚铁毒有关需要进一步研究.本研究旨在通过定位与抗亚铁毒有关的QTL,借助与之紧密连锁的分子标记有效地聚合这些QTL,培育出抗亚铁毒性强的水稻新种质材料.
잠육성수도전엄범분포우중국、사리란잡、인도、인도니서아、새랍리앙、리비아、니일리아、가륜비아화비률빈등국,기중아국남방도구취유근700만공경저산잠육성수도전.해류수도전환원성강,광질영양실조,우이Fe2+과량적루,대수도생장발육산생불량적역경협박작용.배육항아철독적수도품충시간편、경제유효지제고도곡산량적중요도경지일.해문이용유갱도품충Asominori여선도품충IR24잡교연생적Asominori염색체편단치환계(Chromosome Segment Substitution Lines,CSSLs)군체위재료,검측여항아철독협박유관성상QTL.공검측도여항아철독협박유관성상QTL14개,각QTL적LOD치위2.72～6.63.기중검측도여항아철독협박직접유관적성상협편종색반점지수QTL 3개,분별위우제3、9、11염색체C515～XNpb279、R2638～C1263화G1465～C950지간,대응적공헌솔분별위16.45%、11.16%화28.02%;여기타이발표적정위결과비교발현,위우제삼염색체C515～XNpb279간공제협편종색반점지수적QTL여수도공능도보상공제협록소함량적QTL적위치일치;표명재아철독협박조건하,수도재기협편표면출현종색반점,협편쇠로,산생일사협록소강해물혹연생물,이제고협편세포대아철등중금속독해적내수력.령외,재제11염색체G1465～C950지간검측도료공제협편종색반점지수、경간중화근간중QTL 1개,위주효QTL.재제6염색체XNpb386～XNpb342지간검측도공제경간중、주고、근장화근간중QTL 1개,시부여수도항아철독유관수요진일보연구.본연구지재통과정위여항아철독유관적QTL,차조여지긴밀련쇄적분자표기유효지취합저사QTL,배육출항아철독성강적수도신충질재료.
A mapping population of 66 japonica chromosome segment substitution lines (CSSLs) in indica genetic background,derived from a cross between a japonica variety Asominori and an indica variety IR24 by the single-seed descent,backcrossing and marker-assisted selection,was used to detect quantitative trait loci (QTLs) for leaf bronzing index (LBI),stem dry weight (SDW),plant height (PH),root length (RL) and root dry weight (RDW) under Fe2+ stress condition in rice.Two parents and 66 japonica CSSLs were phenotyped for the traits by growing them in Fe2+ toxicity nutrient solution.A total of fourteen QTLs were detected on chromosome 3,6,7,9,11 and 12,respectively,with LOD of QTLs ranging from 2.72 to 6.63.Three QTLs controlling LBI were located at the region of C515～XNpb279,R2638～C1263 and G1465～C950 on chromosome 3,9 and 11,their contributions to whole variation were 16.45%,11.16% and 28.02%,respectively.Comparing with the other mapping results,the QTL for LBI located at the region of C515～XNpb279 on chromosome 3 was identical with the QTL for chlorophyll content on a rice function map.The results indicated that ferrous iron toxicity of rice is characterized by bronzing spots on the lower leaves,which spread over the whole leaves,causing the lower leaves to turn dark gray and to product chlorophyll catabolites or derivatives which reduce cytotoxicity of some heavy metals,such as ferrous iron.Futhermore,the QTL for LBI,SDW and RDW located at the region of G1465～C950 on chromosome 11 is a major QTL.Whether the QTL for SDW ,PH,RL and RDW at the region of XNpb386～XNpb342 on chromosome 6 is associated with resistance to ferrous iron toxicity need further studies.Our goal is to identify breeding materials for resistance to Fe2+ toxicity through marker-assisted selection based on the detected markers.