CAJ | 학술논문

[目的]为优化天然黄酮类物质的提取条件提供一些有益参数.[方法]通过正交试验和方差分析确定了酶提取工艺的最佳参数并将其与常规有机溶剂法进行了比较.[结果]单因素试验表明,当纤维素酶浓度为0.4 mg/ml时,酶解效率最高;pH=4.5时,总黄酮的得率最高;酶解温度为50～55 ℃时,总黄酮的得率较高;酶解2.5 h的总黄酮得率最高.各因素对总黄酮得率的影响大小依次为:pH值>给酶量>酶解时间>酶解温度.通过正交试验和方差分析确定了玉米胚中总黄酮的提取参数为:纤维素酶浓度为0.5 mg/ml,pH=4.5,在50 ℃下酶解3 h,然后在80 ℃下浸提120 min.酶法与有机溶剂法提取总黄酮的平均得率分别为4.65%和2.46%.[结论]酶法提取的提取条件温和,适用于工业生产,完全可以取代常规有机溶剂法.
[목적]위우화천연황동류물질적제취조건제공일사유익삼수.[방법]통과정교시험화방차분석학정료매제취공예적최가삼수병장기여상규유궤용제법진행료비교.[결과]단인소시험표명,당섬유소매농도위0.4 mg/ml시,매해효솔최고;pH=4.5시,총황동적득솔최고;매해온도위50～55 ℃시,총황동적득솔교고;매해2.5 h적총황동득솔최고.각인소대총황동득솔적영향대소의차위:pH치>급매량>매해시간>매해온도.통과정교시험화방차분석학정료옥미배중총황동적제취삼수위:섬유소매농도위0.5 mg/ml,pH=4.5,재50 ℃하매해3 h,연후재80 ℃하침제120 min.매법여유궤용제법제취총황동적평균득솔분별위4.65%화2.46%.[결론]매법제취적제취조건온화,괄용우공업생산,완전가이취대상규유궤용제법.
[ Objective ] The research aimed to supply some useful parameters for optimizing the extraction conditions of natural flavonoids. [Method] The optimum parameters of enzymatic extraction technology were ascertained through orthogonal test and variance analysis and it was compared with conventional organic solvent method. [ Result] It was shown in single factor experiments that when the cellulase concn. was 0.4 mg/ml, the hydrolysis efficiency was highest; when the pH value was 4.5, the yield rate of total flavonoids was highest; when the hydrolysis temperature was 50～55℃, the yield rate of total flavonoids was relatively higher and that of total flavonoids from hydrolysis for 2.5 h was highest. The influences of various factors on the yield rate of total flavonoids were as follows: pH value > enzyme dosage > hydrolysis time > hydrolysis temperature. Through orthogonal test and variance analysis, the extraction parameters of total flavonoids from maize embryos were ascertained as hydrolysis with cellulase concn. of 0.5 mg/ml and pH value of 4.5 under 50 t for 3 h, then extraction for 120 min under 80℃. The average yield rates of total flavonoids extracted by enzymatic method and organic solvent method were 4.65% and 2.46% resp. [ Conclusion ] The extraction conditions of enzymatic extraction were mild and it was suitable for industrial production and could replace conventional organic solvent method completely.