CAJ | 학술논문

Two-dimensional electrophoresis was employed to analyze proteins extracted from sterile and fungal-infected seedling leaves of cucumber S17 at 24 h, 48 h and 72 h after inoculating Sphaerotheca fuliginea. Different spots corresponding to induced or repressed proteins were apparent in Coomassie Brilliant Blue G-250 stained 2-DE gels. Eight different proteins of S17 seedling leaves with qualitative changes or relatively high a-bundance were identified by MALDI-TOF/ TOF and blast in NCBI, including a protein of chloroplast rieske FeS protein, superoxide dismutase [Cu-Zn], chromoplast-specific carotenoid-associated protein, translational-ly controlled tumor protein-related protein, heat shock protein 90, ferredoxin-NADP(H) oxidoreductase, hypothetical protein g5bf and similar to gb |D64087 nuclear matrix constituent protein 1 (NMCP1) from Daucus carota. The proteins induced in response to infection were those involved in disease resistance, heat-shock protein, chromosome transcription and translation, or in protein-related photosynthesis and respiration, as well as some unkown proteins. The results provided new insights for pathogen stress response in cucumber leaves, demonstrating the power of the proteomic approach in plant biology studies.