CAJ | 학술논문

将小麦品种花培3号和豫麦57构建的DH群体的168个株系及其亲本,盆栽于两个环境中,利用324个SSR标记位点构建遗传图谱,对单叶净光合速率及相关参数、叶绿体色素含量和叶绿素荧光参数进行QTL定位和分析.利用基于混合线性模型的QTLNetwork 2.0,共检测到17个加性效应和20对上位性效应位点,其中所有加性效应位点和16对上位性效应位点具有环境互作效应.相关性较高的性状间有一些共同的QTL,表现出一因多效或者紧密连锁效应.在5D染色体上的Xwmc215至Xgdw63区段,检测到控制叶绿素a、叶绿素b和类胡萝卜素含量的3个主效QTL,各位点的遗传效应贡献率较大,增效基因均来源于花培3号,适用于分子标记辅助选择和聚合育种.另外,该区段与控制单叶净光合速率(P_n)、气孔导度(G_s)、胞间CO_2浓度(C_i)和胞间CO_2浓度与胞外CO_2浓度比值(C_i/C_r)的QTL的定位区间相近.位于5B染色体控制胞间CO_2浓度的QTL是个微效基因,但是QTL与两种环境的互作效应表现的遗传贡献比较大.
장소맥품충화배3호화예맥57구건적DH군체적168개주계급기친본,분재우량개배경중,이용324개SSR표기위점구건유전도보,대단협정광합속솔급상관삼수、협록체색소함량화협록소형광삼수진행QTL정위화분석.이용기우혼합선성모형적QTLNetwork 2.0,공검측도17개가성효응화20대상위성효응위점,기중소유가성효응위점화16대상위성효응위점구유배경호작효응.상관성교고적성상간유일사공동적QTL,표현출일인다효혹자긴밀련쇄효응.재5D염색체상적Xwmc215지Xgdw63구단,검측도공제협록소a、협록소b화류호라복소함량적3개주효QTL,각위점적유전효응공헌솔교대,증효기인균래원우화배3호,괄용우분자표기보조선택화취합육충.령외,해구단여공제단협정광합속솔(P_n)、기공도도(G_s)、포간CO_2농도(C_i)화포간CO_2농도여포외CO_2농도비치(C_i/C_r)적QTL적정위구간상근.위우5B염색체공제포간CO_2농도적QTL시개미효기인,단시QTL여량충배경적호작효응표현적유전공헌비교대.
For the purpose of detecting QTLs associated with photosynthetic related traits, a set of 168 doubled haploid (DH) lines derived from the cross between Huapei 3 and Yumai 57 was tested with 324 SSR markers covering the whole genome of wheat (Triticum aestivum L.). The net photosynthetic rate, gas changes, chlorophyll content, and chlorophyll fluorescence in leaves were investigated in both DH population and the parents at seedling stage. QTL analysis was carried out using QTLNetwork version 2.0 based on the mixed linear model. A total of 17 additive QTLs and 20 pairs of epistatic QTLs were detected for the photosyn-thetic related traits. All additive QTLs and 16 pairs of epistatic QTLs had interactions with environments. In agreement with the high correlations of phenotypes, several traits shared common QTL regions, and showed tight linkages of these QTLs or pleiotro-pisms. In the interval between Xwmc215 and Xgdw63 on chromosome 5D, three major additive QTLs for chlorophyll a, chloro-phyll b, and carotinoid contents explained the phenotypic variations by 18.23%, 10.40%, and 27.25%, respectively, whose posi-tive alleles were all originated from Huapei 3. These QTLs are favorable for marker-assisted selection. In addition, this region was near the QTLs for net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO_2 concentration (C_i), and the ratio of C_i to gas conductance (C_r). The QTL for C_i on chromosome 5B was a minor locus but explained relatively great phenotypic variation in the interactions between QTLs and environments.