CAJ | 학술논문

以苯并[a]芘、苯并[a]蒽、苯并[b]荧蒽、苯并[k]荧蒽、茚并[1,2,3-cd]芘5种较难降解的多环芳烃为碳源和能源,采用富集培养的方法从焦化厂污染的土壤中筛选分离得到50株PAHs降解菌.通过对其16S rRNA基因序列分析,将这些PAHs降解菌分为15个种群,分别属于Sphingomonas(鞘氨醇单胞菌属)、Methylobacterium(甲基杆菌属)、Burkholderia(伯克霍尔德氏菌属)、Rhodococcus(红球菌属)、Bradyrhizobium(慢生根瘤菌属)、Phyllobacterium(叶杆菌属)、Chryseobacterium(金黄杆菌属)、Microbacterium(微杆菌属)8个属,其中优势菌为鞘氨醇单胞菌属.纯菌株降解能力测试表明,培养12 d后,菌株3-6-12降解效果要优于其他菌株,对苯并[a]蒽、苯并[a]芘、苯并[b]荧蒽、苯并[k]荧蒽、茚并[1,2,3-cd]芘的降解率分别可达39.64%、33.52%、38.57%、25.37%、31.17%.实验结果可为多环芳烃污染土壤的生物修复提供高效的降解菌源.
이분병[a]비、분병[a]은、분병[b]형은、분병[k]형은、인병[1,2,3-cd]비5충교난강해적다배방경위탄원화능원,채용부집배양적방법종초화엄오염적토양중사선분리득도50주PAHs강해균.통과대기16S rRNA기인서렬분석,장저사PAHs강해균분위15개충군,분별속우Sphingomonas(초안순단포균속)、Methylobacterium(갑기간균속)、Burkholderia(백극곽이덕씨균속)、Rhodococcus(홍구균속)、Bradyrhizobium(만생근류균속)、Phyllobacterium(협간균속)、Chryseobacterium(금황간균속)、Microbacterium(미간균속)8개속,기중우세균위초안순단포균속.순균주강해능력측시표명,배양12 d후,균주3-6-12강해효과요우우기타균주,대분병[a]은、분병[a]비、분병[b]형은、분병[k]형은、인병[1,2,3-cd]비적강해솔분별가체39.64%、33.52%、38.57%、25.37%、31.17%.실험결과가위다배방경오염토양적생물수복제공고효적강해균원.
Microbes play an important role in bioremediation technologies,the aim of this article was to investigate functional strains for soil bio-remediation.In this study benzo[a]pyrene(BaP),benzo[a]anthracene(BaA),benzo[b]fluoranthene(BbF),benzo[k]fluoranthene(BkF),indeno[1,2,3-cd]pyrene(IPY) were used as carbon and energy sources and 50 strains were isolated by steps including enrichment culture and plate cultivation from the contaminated soil at a coking plant in Beijing.The result of 16S rRNA gene sequencing analysis revealed that these strains were clustered into 15 groups and belonged to genera Sphingomonas, Methylobacterium, Burkholderia, Rhodococcus, Bradyrhizobium, Phyllobacterium, Chryseobacterium, Microbacteriumm, of which Sphingomonas was the dominant species.The highest degradation rates of BaA,BaP,BbF,BkF,IPY were 39.64%, 33.52%, 38.57%, 25.37% and 31.17% respectively when tested with strain 3-6-12.The experimental results will be useful for bioremediation of PAHs-contaminated soil.