CAJ | 학술논문

分别以聚马来松香己二醇酯、马来松香乙二醇丙烯酯聚合物和马来松香乙二醇丙烯酯-丙烯酸共聚物为载体,金属离子为桥键配位离子固定化淀粉酶.测定了固定化淀粉酶的性能,探讨了固定化酶反应机理.结果表明金属离子作为桥键配离子的固定化淀粉酶中,PMGAECa(Ⅱ)En和poly(MGAE-AA)Ca(Ⅱ)En效果较好,重复使用5次后,每 5 min 的活性分别为11.33、 9.44 mg/g,相对保留活性为 32.78 %、 37.59 %,最适宜温度为60、 50 ℃,最适宜pH值为 6.03、 5.00.
분별이취마래송향기이순지、마래송향을이순병희지취합물화마래송향을이순병희지-병희산공취물위재체,금속리자위교건배위리자고정화정분매.측정료고정화정분매적성능,탐토료고정화매반응궤리.결과표명금속리자작위교건배리자적고정화정분매중,PMGAECa(Ⅱ)En화poly(MGAE-AA)Ca(Ⅱ)En효과교호,중복사용5차후,매 5 min 적활성분별위11.33、 9.44 mg/g,상대보류활성위 32.78 %、 37.59 %,최괄의온도위60、 50 ℃,최괄의pH치위 6.03、 5.00.
Immobilized amylases were prepared by using polymer of maleated rosin acid-hexandiol ester (PMHE), copolymer of maleated rosin acid-glycol-acrylic acid ester-acrylic acid [poly(MGAE-AA)] and polymer of maleated rosin acid-glycol-acrylic acid ester (PMGAE) respectively as carriers and the method of metallic ion coordination. The performance of the immobilized enzyme was determined. The mechanism of immobilization was discussed. The result showed that PMGAECa(Ⅱ) and poly(MGAE-AA)Ca(Ⅱ) were the best carriers for immobilizing amylase among all carriers with metal ions as coordination bridge. Their activities were maintained at 11.33 and 9.44 mg/g for per 5 min and relative retention activities were 32.78 % and 37.59 %, respectively, after using five times. The optimal reaction conditions of starch oxidation catalyzed by the amylase immobilized on PMGAECa(Ⅱ) and poly(MGAE-AA)Ca(Ⅱ)were found to be as follows: reaction temperature 60 ℃(20 ℃ higher than that of free enzyme) and 50 ℃(10 ℃ higher than that of free enzyme), pH value 6.03 and 5.00, respectively.