中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
1期
50-53
,共4页
曹方方%陈芳%张继红%刘璇%张亚南%张男
曹方方%陳芳%張繼紅%劉璇%張亞南%張男
조방방%진방%장계홍%류선%장아남%장남
神经母细胞瘤%细胞凋亡%NF-κB
神經母細胞瘤%細胞凋亡%NF-κB
신경모세포류%세포조망%NF-κB
Neuroblastoma%Apoptosis%NF-kappa B
目的 探讨小白菊内酯对人神经母细胞瘤细胞系SK-N-SH细胞凋亡的影响及机制.方法 MTT法检测不同浓度小白菊内酯对SK-N-SH细胞增殖抑制的影响,同时计算IC50,根据IC50选取实验组小白菊内酯浓度(10、15μmol/L)；流式细胞仪分析对照组及实验组肿瘤细胞凋亡率；EMSA检测NF-κB的活性；Western Blot法检测细胞凋亡相关蛋白表达.结果 小白菊内酯抑制神经母细胞瘤SK-N-SH细胞生长,并呈浓度依赖关系.用10、15μmol/L小白菊内酯处理细胞48 h后凋亡率分别为(35.63±0.98)％,(53.32±1.08)％,与对照组(6.42±1.26)％比较,差异显著(P＜0.01).EMSA结果表明,小白菊内酯处理组细胞NF-κB活性降低.Western Blot结果显示,小白菊内酯使细胞中Bax、Caspase-3蛋白表达增强,Bcl-2蛋白表达降低.结论 小白菊内酯促进神经母细胞瘤SK-N-SH细胞凋亡,可能与抑制NF-κB活性,下调Bcl-2蛋白表达,上调Bax、Caspase-3蛋白表达有关.
目的 探討小白菊內酯對人神經母細胞瘤細胞繫SK-N-SH細胞凋亡的影響及機製.方法 MTT法檢測不同濃度小白菊內酯對SK-N-SH細胞增殖抑製的影響,同時計算IC50,根據IC50選取實驗組小白菊內酯濃度(10、15μmol/L)；流式細胞儀分析對照組及實驗組腫瘤細胞凋亡率；EMSA檢測NF-κB的活性；Western Blot法檢測細胞凋亡相關蛋白錶達.結果 小白菊內酯抑製神經母細胞瘤SK-N-SH細胞生長,併呈濃度依賴關繫.用10、15μmol/L小白菊內酯處理細胞48 h後凋亡率分彆為(35.63±0.98)％,(53.32±1.08)％,與對照組(6.42±1.26)％比較,差異顯著(P＜0.01).EMSA結果錶明,小白菊內酯處理組細胞NF-κB活性降低.Western Blot結果顯示,小白菊內酯使細胞中Bax、Caspase-3蛋白錶達增彊,Bcl-2蛋白錶達降低.結論 小白菊內酯促進神經母細胞瘤SK-N-SH細胞凋亡,可能與抑製NF-κB活性,下調Bcl-2蛋白錶達,上調Bax、Caspase-3蛋白錶達有關.
목적 탐토소백국내지대인신경모세포류세포계SK-N-SH세포조망적영향급궤제.방법 MTT법검측불동농도소백국내지대SK-N-SH세포증식억제적영향,동시계산IC50,근거IC50선취실험조소백국내지농도(10、15μmol/L)；류식세포의분석대조조급실험조종류세포조망솔；EMSA검측NF-κB적활성；Western Blot법검측세포조망상관단백표체.결과 소백국내지억제신경모세포류SK-N-SH세포생장,병정농도의뢰관계.용10、15μmol/L소백국내지처리세포48 h후조망솔분별위(35.63±0.98)％,(53.32±1.08)％,여대조조(6.42±1.26)％비교,차이현저(P＜0.01).EMSA결과표명,소백국내지처리조세포NF-κB활성강저.Western Blot결과현시,소백국내지사세포중Bax、Caspase-3단백표체증강,Bcl-2단백표체강저.결론 소백국내지촉진신경모세포류SK-N-SH세포조망,가능여억제NF-κB활성,하조Bcl-2단백표체,상조Bax、Caspase-3단백표체유관.
Objective To explore the effects of parthenolide on proliferation and apoptosis of human neuroblastoma cell line SK-N-SH.Methods SK-N-SH,a human neuroblastoma cell line,was treated with different concentrations of parthenolide for 48h,the cellular proliferative activities were examined by MTT assay.The parthenolide concentration of experimental group (10 mol/L,15 mol/L)was determined by IC50.Apoptosis rate of SK-N-SH cells was analyzed by flow cytometry (FCM).The activity of NF-κB of SK-N-SH cells after parthenolide treatment was assayed by EMSA.WesternBlot was used to detect protein expression of Bcl-2,Bax,Caspase-3.Results The proliferative activities of SK-N-SH treated with parthenolide were obviously suppressed,the proliferative rate decreased in a dose-dependent manner.Treated with 10 mol/L and 15 mol/L parthenolide after 48h,the apoptosis rate was 35.63 ± 0.98％ and 53.32 ± 1.08％,respectively.Compared with the control group (6.42 ±1.26％),the differences were significant (P＜0.01).EMSA results showed that the activity of NF-κB in SK-N-SH cells after parthenolide treatment for 48h was decreased.Western Blot showed that parthenolide increased protein levels of Bax and Caspase-3 and decreased protein level of Bcl-2 in SK-NSH cells.Conclusions Parthenolide can suppress the proliferative activity of neuroblastoma cell line SK-N-SH and induce cell apoptosis.The anti-tumor mechanism of parthenolide may be related to the inhibited NF-κB,reduced Bcl-2 protein expression and increased Bax,Caspase-3 proteins.
