CAJ | 학술논문

目的观察1-甲基色氨酸(1-MT)对荷肝癌小鼠皮下移植瘤生长的抑制作用.方法建立荷肝癌小鼠皮下移植瘤模型.实验分为hepG2组、空质粒组、吲哚胺2,3双加氧酶(IDO)生理盐水组、IDO 5-氟尿嘧啶(5-FU)组、IDO 1-MT组、IDO 1-MT和5-FU联合处理组,每组各8只.观测各组小鼠成瘤情况肿瘤体积差异以及常规病理检查变化,并采用免疫组化法检测肿瘤组织内IDO表达情况.结果与hepG2和空质粒生理盐水组比较,IDO生理盐水组肿瘤体积大、生长快(P＜0.05).与IDO生理盐水组比较,5-FU组、1-MT组和联合处理组肿瘤体积较小、重量减轻,其抑瘤率分别为86.54％、79.95％、94.46％,差异有统计学意义(P＜0.05).其中联合用药组效果最好(P＜0.05),5-FU组与1-MT组两者间肿瘤体积变化无显著差异(P＞0.05).HE病理切片观察各处理组可见癌细胞密度减少,坏死区域增加.各处理组外周血甲胎蛋白(AFP)降低,差异有统计学意义(P＜0.05).结论 IDO可促进肝癌细胞的生长,参与了肝癌细胞的免疫逃逸；1-MT对人肝癌小鼠皮下移植瘤生长具有抑制作用,与化疗药联合应用效果更好.
목적 관찰1-갑기색안산(1-MT)대하간암소서피하이식류생장적억제작용.방법 건립하간암소서피하이식류모형.실험분위hepG2조、공질립조、신타알2,3쌍가양매(IDO)생리염수조、IDO 5-불뇨밀정(5-FU)조、IDO 1-MT조、IDO 1-MT화5-FU연합처리조,매조각8지.관측각조소서성류정황종류체적차이이급상규병리검사변화,병채용면역조화법검측종류조직내IDO표체정황.결과 여hepG2화공질립생리염수조비교,IDO생리염수조종류체적대、생장쾌(P＜0.05).여IDO생리염수조비교,5-FU조、1-MT조화연합처리조종류체적교소、중량감경,기억류솔분별위86.54％、79.95％、94.46％,차이유통계학의의(P＜0.05).기중연합용약조효과최호(P＜0.05),5-FU조여1-MT조량자간종류체적변화무현저차이(P＞0.05).HE병리절편관찰각처리조가견암세포밀도감소,배사구역증가.각처리조외주혈갑태단백(AFP)강저,차이유통계학의의(P＜0.05).결론 IDO가촉진간암세포적생장,삼여료간암세포적면역도일；1-MT대인간암소서피하이식류생장구유억제작용,여화료약연합응용효과경호.
Objective To investigate the inhibitory effect of 1-methyl-tryptophan (1-MT) on transplanted hepatocellular carcinoma in mice.Methods Human hepatocellular carcinoma subcutaneous tumor models were established in mice,and the mice were divided into hepG2 group,empty plasmid group,indoleamine 2,3-dioxygenase (IDO) saline group,IDO) 5-fluoropyrimidine (5-FU) group,IDO 1-MT group,and the group combining IDO 1-MT with 5-FU treatment (n=8 in each group).The tumor growth,tumor volume and pathological examination were observed and the expression of IDO in tumor tissues was determined by immunohistochemistry.Results Compared with hepG2 and the empty vector saline groups,IDO saline group had bigger tumor,faster growth,and the differences were statistically significant (P＜0.05).Compared with IDO saline group,5-FU group,1-MT group and combination treatment groups showed smaller tumor volume and weight,and the tumor inhibitory rates were 86.54％,79.95％,94.46％,respectively.There were significant differences between these groups (P＜0.05).However,there were no significant differences in tumor volumes between 5-FU group and 1-MT group (P＞0.05).HE pathological observation of ceils in each treatment group showed reduced density,increased necrotic area and significant decrease in peripheral blood alpha-fetoprotein (P＜0.05).Conclusions IDO can promote the growth of liver cancer cells involved in immune escape.1-MT can inhibit the transplanted tumor growth in mice,and therefore may enhance the chemotherapeutic efficacy.