中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
8期
449-454
,共6页
李文渊%叶超%林镯%洪巧%王柯尹%卢明芹%陈永平
李文淵%葉超%林鐲%洪巧%王柯尹%盧明芹%陳永平
리문연%협초%림탁%홍교%왕가윤%로명근%진영평
内毒素类%肝功能衰竭,急性%白细胞介素1受体相关激酶类%NF-κB
內毒素類%肝功能衰竭,急性%白細胞介素1受體相關激酶類%NF-κB
내독소류%간공능쇠갈,급성%백세포개소1수체상관격매류%NF-κB
Endotoxins%Liver failure,acute%Interleukin-1 receptor associated kinases%NFkappa B
目的 探讨内毒素耐受(ETT)大鼠肝组织IL-1受体相关激酶(IRAK)-4和IRAK-M的表达变化及其意义.方法 将66只雄性SD大鼠分为对照组、急性肝功能衰竭(ALF)组和ETT组.ETT组腹腔注射脂多糖(LPS),ALF组注射等量0.75%氯化钠溶液,然后ETT组与ALF组同时腹腔注射D-氨基半乳糖(D-GalN)及LPS,在注射后2、6、12、24和48 h时间点留取大鼠血液及肝脏标本.观察肝组织的病理变化,ELISA法检测TNF-a及IL-6水平,RT-PCR检测IRAK-4、IRAKM、核因子-κB(NF-κB)(p65)mRNA表达情况.统计学处理采用LSD检验和Dunnet's t检验.结果 ETT组大鼠肝组织病理改变明显轻于ALF组.在2、6、12、24、48 h,ALF组大鼠肝组织IRAK-4mRNA/β-肌动蛋白(β-actin)吸光度比值分别为0.711±0.074、0.904±0.118、1.012±0.098、1.534±0.279、1.451±0.290,IRAK-M mRNA/β-actin吸光度比值分别为0.496±0.018、0.516±0.089、0.503±0.023、0.503±0.057、0.469±0.142;ETT组IRAK-M mRNA/β-actin吸光度比值分别为0.929±0.064、1.111±0.138、1.113±0.027、1.891±0.315、1.710±0.303,IRAK-4 mRNA/β-actin吸光度比值分别为0.619±0.083、0.587±0.033、0.623±0.034、0.720±0.044、0.654±0.041.与ALF组比较,ETT组IRAK-M mRNA表达明显上升,IRAK-4 mRNA表达相对降低,两组IRAK-4、NF-κB(p65)及IRAK-M在2、6、12、24、48 h均差异有统计学意义(F=17.305、54.921、121.031、67.607、55.279,F=19.506、43.777、110.823、302.681、202.822,F=172.003、59.519、987.055、68.463、96.601,均P＜0.05).结论 诱导ETT可使ALF大鼠肝组织损害减轻,可能与IRAK-4表达下降、IRAK-M表达升高有关.
目的 探討內毒素耐受(ETT)大鼠肝組織IL-1受體相關激酶(IRAK)-4和IRAK-M的錶達變化及其意義.方法 將66隻雄性SD大鼠分為對照組、急性肝功能衰竭(ALF)組和ETT組.ETT組腹腔註射脂多糖(LPS),ALF組註射等量0.75%氯化鈉溶液,然後ETT組與ALF組同時腹腔註射D-氨基半乳糖(D-GalN)及LPS,在註射後2、6、12、24和48 h時間點留取大鼠血液及肝髒標本.觀察肝組織的病理變化,ELISA法檢測TNF-a及IL-6水平,RT-PCR檢測IRAK-4、IRAKM、覈因子-κB(NF-κB)(p65)mRNA錶達情況.統計學處理採用LSD檢驗和Dunnet's t檢驗.結果 ETT組大鼠肝組織病理改變明顯輕于ALF組.在2、6、12、24、48 h,ALF組大鼠肝組織IRAK-4mRNA/β-肌動蛋白(β-actin)吸光度比值分彆為0.711±0.074、0.904±0.118、1.012±0.098、1.534±0.279、1.451±0.290,IRAK-M mRNA/β-actin吸光度比值分彆為0.496±0.018、0.516±0.089、0.503±0.023、0.503±0.057、0.469±0.142;ETT組IRAK-M mRNA/β-actin吸光度比值分彆為0.929±0.064、1.111±0.138、1.113±0.027、1.891±0.315、1.710±0.303,IRAK-4 mRNA/β-actin吸光度比值分彆為0.619±0.083、0.587±0.033、0.623±0.034、0.720±0.044、0.654±0.041.與ALF組比較,ETT組IRAK-M mRNA錶達明顯上升,IRAK-4 mRNA錶達相對降低,兩組IRAK-4、NF-κB(p65)及IRAK-M在2、6、12、24、48 h均差異有統計學意義(F=17.305、54.921、121.031、67.607、55.279,F=19.506、43.777、110.823、302.681、202.822,F=172.003、59.519、987.055、68.463、96.601,均P＜0.05).結論 誘導ETT可使ALF大鼠肝組織損害減輕,可能與IRAK-4錶達下降、IRAK-M錶達升高有關.
목적 탐토내독소내수(ETT)대서간조직IL-1수체상관격매(IRAK)-4화IRAK-M적표체변화급기의의.방법 장66지웅성SD대서분위대조조、급성간공능쇠갈(ALF)조화ETT조.ETT조복강주사지다당(LPS),ALF조주사등량0.75%록화납용액,연후ETT조여ALF조동시복강주사D-안기반유당(D-GalN)급LPS,재주사후2、6、12、24화48 h시간점류취대서혈액급간장표본.관찰간조직적병리변화,ELISA법검측TNF-a급IL-6수평,RT-PCR검측IRAK-4、IRAKM、핵인자-κB(NF-κB)(p65)mRNA표체정황.통계학처리채용LSD검험화Dunnet's t검험.결과 ETT조대서간조직병리개변명현경우ALF조.재2、6、12、24、48 h,ALF조대서간조직IRAK-4mRNA/β-기동단백(β-actin)흡광도비치분별위0.711±0.074、0.904±0.118、1.012±0.098、1.534±0.279、1.451±0.290,IRAK-M mRNA/β-actin흡광도비치분별위0.496±0.018、0.516±0.089、0.503±0.023、0.503±0.057、0.469±0.142;ETT조IRAK-M mRNA/β-actin흡광도비치분별위0.929±0.064、1.111±0.138、1.113±0.027、1.891±0.315、1.710±0.303,IRAK-4 mRNA/β-actin흡광도비치분별위0.619±0.083、0.587±0.033、0.623±0.034、0.720±0.044、0.654±0.041.여ALF조비교,ETT조IRAK-M mRNA표체명현상승,IRAK-4 mRNA표체상대강저,량조IRAK-4、NF-κB(p65)급IRAK-M재2、6、12、24、48 h균차이유통계학의의(F=17.305、54.921、121.031、67.607、55.279,F=19.506、43.777、110.823、302.681、202.822,F=172.003、59.519、987.055、68.463、96.601,균P＜0.05).결론 유도ETT가사ALF대서간조직손해감경,가능여IRAK-4표체하강、IRAK-M표체승고유관.
Objective To explore the dynamic expressions of interleukin-1 (IL-1)receptor associated kinase (IRAK)-M and IRAK-4 in rats with or without endotoxin tolerance (ETT)in acute liver failure (ALF).Methods Sixty-six male SD rats were divided into three groups:ALF group,ETT group and control group.The rats in ETT group received daily lipopolysaccharide (LPS)intraperitoneal injection for 5 days,while the rats in ALF group received daily injection with same volume of 0.75% NaCl solution.Both ETT group and ALF group received intraperitoneal injection with D-galactosamine (D-GalN)and LPS at 24 hours after the 5th injection of LPS or NaCl solution.At 2,6,12,24 and 48 h after D-GalN and LPS injection,the serum levels of tumor necrosis factor-α (TNF-α)and interleukin-6 (IL-6)were detected by enzyme-linked immunosorbent assay (ELISA).The liver pathologic changes were observed with HE staining by microscope.The mRNA expressions of IRAK-4,IRAK-M and NF-κB (p65)were detected by reverse transcriptase polymerase chain reaction (RT-PCR).The pairwise comparison between groups was done by lease significant difference (LSD)and Dunnet's t test.Results The liver pathologic changes in ETT group were much milder than those of ALF group.In ALF group,IRAK-4 mRNA/β-actin absorbance ratios at 2,6,12,24and 48 h after D-GalN and LPS challenge were 0.711 ±0.074,0.904±0.118,1.012 ±0.098,1.534±0.279 and 1.451±0.290,respectively,while the IRAK-M mRNA/β-actin absorbance ratios were 0.496±0.018,0.516±0.089,0.503±0.023,0.503±0.057 and 0.469±0.142,respectively.In ETT group,the IRAK-4 mRNA/β-actin absorbance ratios at 2,6,12,24 and 48 h after D-GalN and LPS challenge were 0.619±0.083,0.587±0.033,0.623±0.034,0.720±0.044 and 0.654±0.041,respectively,while the IRA'K-M mRNA/β-actin absorbance ratios were 0.929 ± 0.064,1.111±0.138,1.113±0.027,1.891±0.315 and 1.710±0.303,respectively.The IRAK-M mRNA expression level was significantly increased and IRAK-4 mRNA expression level was relatively decreased in ETT group compared to ALF group.The differences were all statistically significant at 2,6,12,24 and 48 h after D-GalN and LPS challenge (F= 17.305,54.921,121.031,67.607,55.279,respectively; F=19.506,43.777,110.823,302.681,202.822,respectively; F=172.003,59.519,987.055,68.463,96.601,respectively; all P＜0.05).Conclusion LPS pretreatment may induce ETT in rat model by downregulating the expression of IRAK-4 and upregulating the expression of IRAK-M.
