CAJ | 학술논문

目的研究非人灵长类动物猴腺病毒的分子多样性。方法采集圈养的57只恒河猴粪便样本，以腺病毒聚合酶基因为目的基因，用PCR方法进行扩增，对PCR阳性产物进行克隆、测序，并进行系统进化分析。结果 57只恒河猴粪便样本有12份样本中存在腺病毒DNA，系统进化分析提示这些序列主要可分为两大组：类SAdV-6组（2个非重复序列）和类SAdV-7组（9个非重复序列）。此外，有三个克隆，其最相似序列分别为：SAdV-1,SAdV-3和HAdV-52。结论我们的研究证实了腺病毒的确在非人灵长类粪便中普遍存在，揭示了本研究所涉及动物肠道内腺病毒的多样性以及系统进化情况。
목적 연구비인령장류동물후선병독적분자다양성。방법 채집권양적57지항하후분편양본，이선병독취합매기인위목적기인，용PCR방법진행확증，대PCR양성산물진행극륭、측서，병진행계통진화분석。결과 57지항하후분편양본유12빈양본중존재선병독DNA，계통진화분석제시저사서렬주요가분위량대조：류SAdV-6조（2개비중복서렬）화류SAdV-7조（9개비중복서렬）。차외，유삼개극륭，기최상사서렬분별위：SAdV-1,SAdV-3화HAdV-52。결론 아문적연구증실료선병독적학재비인령장류분편중보편존재，게시료본연구소섭급동물장도내선병독적다양성이급계통진화정황。
Objective In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate (NHP) populations. MethodsWe examined a colony of captively bred rhesus macaques (Macaca mulatta ) in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses. ResultsAmong the 57animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA. The results suggested that the viral DNA clones were primarily segregated into two large groups: SAdV-6 (2 nonredundant sequences) and SAdV-7 (9 non-redundant sequences). In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively. ConclusionOur data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.