CAJ | 학술논문

目的探讨尿液抗增殖因子(APF)活性测定在间质性膀胱炎(IC)诊疗中的价值.方法收集25例IC患者作为治疗组和25例输尿管结石患者作为对照组.采用膀胱扩张及膀胱灌注西施泰对IC患者治疗6个月,留取治疗前、治疗后3个月和6个月尿液并行盆腔疼痛和尿急/尿频症状评分(PUF).采用角蛋白抗体对膀胱上皮细胞进行免疫细胞化学染色法的鉴定.应用噻唑蓝(MTT)比色法检测治疗前后尿液对正常膀胱上皮细胞的抑制活性.结果对照组中尿液对正常膀胱上皮细胞没有抑制活性.治疗组治疗前后尿液APF活性比较,差异有统计学意义(F=396.798,P＜0.05);两两比较显示术前同术后3个月和6个月差异有统计学意义(P＜0.05).PUF评分治疗前后比较,差异有统计学意义(F=50.812,P＜0.05);两两比较显示术前同术后3个月和6个月差异有统计学意义(P＜0.05).术前APF同PUF(rs=0.974,P＜0.05);术后3个月APF同PUF(rs=0.836,P＜0.05);术后6个月APF同PUF(rs=0.679,P＜0.05).结论 APF活性同PUF评分呈正相关,尿液APF活性测定可以作为IC诊断及疗效评价的客观指标.
목적 탐토뇨액항증식인자(APF)활성측정재간질성방광염(IC)진료중적개치.방법 수집25례IC환자작위치료조화25례수뇨관결석환자작위대조조.채용방광확장급방광관주서시태대IC환자치료6개월,류취치료전、치료후3개월화6개월뇨액병행분강동통화뇨급/뇨빈증상평분(PUF).채용각단백항체대방광상피세포진행면역세포화학염색법적감정.응용새서람(MTT)비색법검측치료전후뇨액대정상방광상피세포적억제활성.결과 대조조중뇨액대정상방광상피세포몰유억제활성.치료조치료전후뇨액APF활성비교,차이유통계학의의(F=396.798,P＜0.05);량량비교현시술전동술후3개월화6개월차이유통계학의의(P＜0.05).PUF평분치료전후비교,차이유통계학의의(F=50.812,P＜0.05);량량비교현시술전동술후3개월화6개월차이유통계학의의(P＜0.05).술전APF동PUF(rs=0.974,P＜0.05);술후3개월APF동PUF(rs=0.836,P＜0.05);술후6개월APF동PUF(rs=0.679,P＜0.05).결론 APF활성동PUF평분정정상관,뇨액APF활성측정가이작위IC진단급료효평개적객관지표.
Objective To approach the value of antiproliferative factor (APF) activity in the diagnosis and treatment of interstitial cystitis (IC). Methods Sterilitas urine specimens were harvested from 25 female patients with IC and 25 female normal controls. IC patients were treated with hydrodistention and subjected to irrigation of bladder with cystistat for 6 months. Sterilitas urine specimens and pelvic pain and urgency/frequency patient symptom scale (PUF) in IC group were determined before, and 3 and 6 months after treatment. Immunohistochemistry with AE1/AE3 was used to identify human bladder epithelial cells.Methylthiazol tetrazolium (MTT) assay was used to detect APF activity in patients with IC. Results There was significant difference in APF activity(F=396.798) and PUF (F=50.812) before and after treatment. Before treatment APF activity/PUF were significantly higher than those at the 3rd and 6th month post-treatment. There was a significant positive correlation between APF activity and PUF before and after treatment (rs =0.974, rs=0.836, rs=0.679). Conclusion There ia a significant positive correlation between APF activity and PUF. Urine APF activity can be used as an objective marker to diagnose IC and evaluate the curative effectiveness.