中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1379-1382
,共4页
王辛%柳晓义%曹伟红%李福年
王辛%柳曉義%曹偉紅%李福年
왕신%류효의%조위홍%리복년
白细胞介素-8%CXCR2%基因多态性%乳腺癌
白細胞介素-8%CXCR2%基因多態性%乳腺癌
백세포개소-8%CXCR2%기인다태성%유선암
Interleukin-8%CXCR2%Single nucleotide polymorphism%Breast carcinoma
目的 探讨细胞因子白细胞介素( 1L)-8(- 251)位点及其受体CXCR2(±1208)位点多态性与乳腺癌发病风险及侵袭特性的关系.方法 采用等位基因特异-聚合酶链反应(AS-PCR)分析方法检测228名乳腺癌患者和100例健康对照者的IL-8(- 251)位点及其受体CXCR2(±1208)位点多态性分布,并进行统计学分析.结果 IL-8(- 251)位点TA型和AA型以及CXCR2(± 1208) TT型是乳腺癌的高危基因型[比值比(OR)=1.57,95％可信区间(CI)=1.08～2.32；OR =2.68,95％ CI=1.26 ～2.99；0R=2.02,95％CI=1.08～3.62],在两组间表达频率的差异有统计学意义(P＜0.05).携带1个及以上高危基因型增加患乳腺癌的风险.分层分析表明携带IL-8(-251)等位基因A和CXCR2(± 1208)等位基因T增加乳腺癌的侵袭特性,其分布频率在肿瘤的高组织学分级(OR=1.95,P＜0.01;OR=1.52,P＜0.05)和淋巴结转移阳性(OR=1.65,P＜0.05；0R=1.66,P＜0.01)中差异有统计学意义.另外,IL-8(-251)A等位基因与ER( -)乳腺癌显著相关( OR=1.65,P＜0.05).结论 IL-8和CXCR2的基因多态性可能与女性乳腺癌的发生发展关系密切,可作为乳腺癌早期基因诊断的指标.
目的 探討細胞因子白細胞介素( 1L)-8(- 251)位點及其受體CXCR2(±1208)位點多態性與乳腺癌髮病風險及侵襲特性的關繫.方法 採用等位基因特異-聚閤酶鏈反應(AS-PCR)分析方法檢測228名乳腺癌患者和100例健康對照者的IL-8(- 251)位點及其受體CXCR2(±1208)位點多態性分佈,併進行統計學分析.結果 IL-8(- 251)位點TA型和AA型以及CXCR2(± 1208) TT型是乳腺癌的高危基因型[比值比(OR)=1.57,95％可信區間(CI)=1.08～2.32；OR =2.68,95％ CI=1.26 ～2.99；0R=2.02,95％CI=1.08～3.62],在兩組間錶達頻率的差異有統計學意義(P＜0.05).攜帶1箇及以上高危基因型增加患乳腺癌的風險.分層分析錶明攜帶IL-8(-251)等位基因A和CXCR2(± 1208)等位基因T增加乳腺癌的侵襲特性,其分佈頻率在腫瘤的高組織學分級(OR=1.95,P＜0.01;OR=1.52,P＜0.05)和淋巴結轉移暘性(OR=1.65,P＜0.05；0R=1.66,P＜0.01)中差異有統計學意義.另外,IL-8(-251)A等位基因與ER( -)乳腺癌顯著相關( OR=1.65,P＜0.05).結論 IL-8和CXCR2的基因多態性可能與女性乳腺癌的髮生髮展關繫密切,可作為乳腺癌早期基因診斷的指標.
목적 탐토세포인자백세포개소( 1L)-8(- 251)위점급기수체CXCR2(±1208)위점다태성여유선암발병풍험급침습특성적관계.방법 채용등위기인특이-취합매련반응(AS-PCR)분석방법검측228명유선암환자화100례건강대조자적IL-8(- 251)위점급기수체CXCR2(±1208)위점다태성분포,병진행통계학분석.결과 IL-8(- 251)위점TA형화AA형이급CXCR2(± 1208) TT형시유선암적고위기인형[비치비(OR)=1.57,95％가신구간(CI)=1.08～2.32；OR =2.68,95％ CI=1.26 ～2.99；0R=2.02,95％CI=1.08～3.62],재량조간표체빈솔적차이유통계학의의(P＜0.05).휴대1개급이상고위기인형증가환유선암적풍험.분층분석표명휴대IL-8(-251)등위기인A화CXCR2(± 1208)등위기인T증가유선암적침습특성,기분포빈솔재종류적고조직학분급(OR=1.95,P＜0.01;OR=1.52,P＜0.05)화림파결전이양성(OR=1.65,P＜0.05；0R=1.66,P＜0.01)중차이유통계학의의.령외,IL-8(-251)A등위기인여ER( -)유선암현저상관( OR=1.65,P＜0.05).결론 IL-8화CXCR2적기인다태성가능여녀성유선암적발생발전관계밀절,가작위유선암조기기인진단적지표.
Objective To explore the association between interleukin (IL)-8 gene -251 A/T and CXCR2 gene ± 1208 C/T polymorphisms with the risk and aggressive forms of breast cancer.Methods Totally 228 breast cancer patients and 100 non-cancer female controls were selected.The genotypes were detected by using AS-PCR method.Results IL-8 ( -251 ) TA/AA and CXCR2 ( ± 1208 ) TT were the high risk genotypes ( OR =1.57,95％ CI =1.08-2.32 ; OR =2.68,95％ CI =1.26-2.99 ; OR =2.02,95％ CI =1.08-3.62 ),and there was significant difference in the expression distributation between the case group and the control group.The risk of breast carcinoma was significantly in the patients carrying one high-risk genotype and above7.The stratified analysis indicated that IL-8 ( - 251 ) A allele and CXCR2 ( ± 1208) T allele are highly associated with aggressive forms of breast cancer as denied by high grade and lymph node metastases (OR =1.65,95％ CI =1.21-2.74; OR =1.66,95％ CI =1.17-2.73 ).Conclusion The results indicated that the polymorphisms in IL-8 and CXCR2 genes,which might be associated with breast cancer risk of Chinese women,as well as disease progress,could be used as an indicator of the early genetic diagnosis of breast cancer.
