CAJ | 학술논문

目的探讨黄岑苷对人肺腺癌A549细胞株增殖影响的分子机制.方法体外培养人肺腺癌细胞株A549,加入0.000、0.625、1.250、2.500、5.000、10.000 mg/L黄芩苷,分别孵育24、48、72 h,噻唑蓝(MTT)法检测药物对A549细胞的抑制率；1.22 mg/L黄芩苷孵育A549细胞24 h后,收集细胞,逆转录-聚合酶链反应( RT-PCR)测定增殖细胞核抗原(PCNA)、细胞周期蛋白 1(Cyclin D1) 、p21 mRNA的表达水平；Western blot法检测PCNA、Cyclin D1、p21蛋白表达水平.结果 MTT结果显示黄芩苷对人肺腺癌A549细胞株增殖具有明显的抑制作用.1.22 mg/L黄芩苷作用细胞24 h后,与对照组(0.68±0.16、0.48±0.05、0.27±0.06)比较,PCNA mRNA表达(0.22±0.03)、Cvclin D1 mRNA表达(0.08±0.02)显著下降(P＜0.01),p21 mRNA表达(0.47±0.07)显著升高(P＜0 01) 与对照组(1.75±0.05,1.59 ±0.24,0.59±0.02)比较,黄芩苷组PCNA蛋白表达(0.34±0.02)、Cvclin D1蛋白表达(0.50±0.02)显著下降(P＜0.01),p21蛋白表达(1.39±0.05)显著升高(P＜0.01).结论黄芩苷可通过下调PCNA、Cyclin D1表达,上调p21表达,发挥其抑制A549肺腺癌细胞增殖的作用.
목적 탐토황잠감대인폐선암A549세포주증식영향적분자궤제.방법 체외배양인폐선암세포주A549,가입0.000、0.625、1.250、2.500、5.000、10.000 mg/L황금감,분별부육24、48、72 h,새서람(MTT)법검측약물대A549세포적억제솔；1.22 mg/L황금감부육A549세포24 h후,수집세포,역전록-취합매련반응( RT-PCR)측정증식세포핵항원(PCNA)、세포주기단백 1(Cyclin D1) 、p21 mRNA적표체수평；Western blot법검측PCNA、Cyclin D1、p21단백표체수평.결과 MTT결과현시황금감대인폐선암A549세포주증식구유명현적억제작용.1.22 mg/L황금감작용세포24 h후,여대조조(0.68±0.16、0.48±0.05、0.27±0.06)비교,PCNA mRNA표체(0.22±0.03)、Cvclin D1 mRNA표체(0.08±0.02)현저하강(P＜0.01),p21 mRNA표체(0.47±0.07)현저승고(P＜0 01) 여대조조(1.75±0.05,1.59 ±0.24,0.59±0.02)비교,황금감조PCNA단백표체(0.34±0.02)、Cvclin D1단백표체(0.50±0.02)현저하강(P＜0.01),p21단백표체(1.39±0.05)현저승고(P＜0.01).결론 황금감가통과하조PCNA、Cyclin D1표체,상조p21표체,발휘기억제A549폐선암세포증식적작용.
Objective To investigate the eftects of baicalin on proliferation of cells-line-AS49 and its action mechanism.Methods Cultured A549 cells were treated with baicalin (0.000,0.625,1.250,2.500,5.000,10.000 mg/L) for 24,48 and 72 h,then inhibition rate was measured by methyl thiazol tetrazolium (MTT) assay.Cells treated with 1.22 mg/L baicalin for 24 h were collected and subjected to reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting for the expression levels of proliferating cell nuclear antigen (PCNA),Cyclin D1 and p21.Results MTT indicated that the proliferation of A549 cells was inhibited by baicalin at different concentrations.As compared with control group (0.68 ±0.16,0.48 ±0.05,0.27 ±0.06),PCNA mRNA (0.22 ±0.03) abd Cyclin D1 mRNA (0.08 ± 0.02) were down-regulated,and p21 mRNA (0.47 ± 0.07 ) was up-regulated markedly by 1.22 mg/L baicalin for 24 h. As compared with control group (1.75 ±0.05,1.59 ±0.24,0.59±0.02),PCNA protein (0.34 ± 0.02 ) and Cyclin D1 protein (0.50 ± 0.02 ) were down-regulated,and p21 protein (1.39 ± 0.05) was up-regulated by baicalin.Conclusion Baicalin inhibits A549 cells proliferation by down-regulating the expression of PCNA and Cyclin D l,and by up-regulating the expression of p21.