CAJ | 학술논문

目的探讨乳腺癌组织中缺氧诱导因子-1α(HIF-1α)和葡萄糖转运蛋白1(Glut1)表达的关联性及其与细胞增生活性和临床病理因素的关系.方法采用免疫组织化学方法检测HIF-1α、Glutl、增生细胞核抗原(PCNA)在80例乳腺癌组织、20例乳腺纤维腺瘤及20例乳腺增生组织中的表达水平并比较它们之间的相关性.结果 HIF-1α和Glut1在乳腺纤维腺瘤、乳腺增生组织中不表达;HIF-1α在乳腺导管内原位癌中阳性率55.0%(11/20),浸润性乳腺癌中85.0%(51/60);乳腺癌中Glut1阳性率58.8%(47/80);乳腺癌中PCNA阳性率75%(60/80),其中原位癌65%(13/20),浸润癌78.3%(47/60).乳腺癌中HIF-1α表达与GIut1呈显著正相关(r=0.653,P＜0.01);HIF-1α表达与PCNA呈显著正相关(r=0.693,P＜0.01);Glut1与PCNA呈显著正性相关(r=0.742,P＜0.01).HIF-1α和Ght1在淋巴结、雌激素受体状态及组织学分级中表达差异有统计学意义;Glutl在肿瘤大小及孕激素受体状态表达差异也有统计学意义.结论 HIF-1α和Giutl蛋白的过表达共同参与了乳腺癌的发生、发展,与乳腺癌细胞增生活性密切相关,有望成为乳腺癌治疗的新靶点.
목적 탐토유선암조직중결양유도인자-1α(HIF-1α)화포도당전운단백1(Glut1)표체적관련성급기여세포증생활성화림상병리인소적관계.방법 채용면역조직화학방법검측HIF-1α、Glutl、증생세포핵항원(PCNA)재80례유선암조직、20례유선섬유선류급20례유선증생조직중적표체수평병비교타문지간적상관성.결과 HIF-1α화Glut1재유선섬유선류、유선증생조직중불표체;HIF-1α재유선도관내원위암중양성솔55.0%(11/20),침윤성유선암중85.0%(51/60);유선암중Glut1양성솔58.8%(47/80);유선암중PCNA양성솔75%(60/80),기중원위암65%(13/20),침윤암78.3%(47/60).유선암중HIF-1α표체여GIut1정현저정상관(r=0.653,P＜0.01);HIF-1α표체여PCNA정현저정상관(r=0.693,P＜0.01);Glut1여PCNA정현저정성상관(r=0.742,P＜0.01).HIF-1α화Ght1재림파결、자격소수체상태급조직학분급중표체차이유통계학의의;Glutl재종류대소급잉격소수체상태표체차이야유통계학의의.결론 HIF-1α화Giutl단백적과표체공동삼여료유선암적발생、발전,여유선암세포증생활성밀절상관,유망성위유선암치료적신파점.
Objective To investigate the expression of hypoxia-inducible 1 alpha(HIF-lα)and glucose transporter 1(Glut1)in human breast cancer and its relationship to proliferating cell nuclear antigen (PCNA)protein and clinical pathologic factors.Methods Immunohistochemical staining was used to measure the expression of HIF-lα.Glut1 and PCNA in human breast fibroadenoma,usual hyperplasia and breast carcinoma.Results HIF-1α expression was not found in breast fibroadenoma and hyperplastic Iesions.In contrast.the positive rate of HIF-1α was found in the ductal carcinoma in situ 55%(DCIS,11/20)and the invasive breast carcinoma 85%(51/60).Glut1 positivity in breast carcinoma was 58.8%(47/80).The totsl positive rate of PCNA in breast carcinoma was 75%(60/80),that in DCIS was 65%(13/20)and that in invasive carcinoma was 78.3%(47/60).There was a positive correlation between HIF-lα and Glut 1 level (r=0.653,P＜0.01),a positive correlation between HIF-1α and PCNA level(r=0.693,P＜0.01);and also a positive correlation between Glutl and PCNA level(t=0.742.P＜0.01).conclusion The overexpression of HIF-lα and its target gene Glut1 played important roles in carcinogenesis and progression of breast carcinoma and closely correlated with cell proliferation of breast carcinoma and may become a new target for treatment of breast carcinoma.