肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
10期
665-668
,共4页
李宜放%王晞星%刘丽坤%汪欣文%刘改萍%王惠媛
李宜放%王晞星%劉麗坤%汪訢文%劉改萍%王惠媛
리의방%왕희성%류려곤%왕흔문%류개평%왕혜원
肠瑞灌肠剂%放射性%直肠炎%白细胞介素1β%核因子-κB%反转录聚合酶链反应%免疫组织化学
腸瑞灌腸劑%放射性%直腸炎%白細胞介素1β%覈因子-κB%反轉錄聚閤酶鏈反應%免疫組織化學
장서관장제%방사성%직장염%백세포개소1β%핵인자-κB%반전록취합매련반응%면역조직화학
Changrui enemas%Radioactivity%Proctitis%Interleukin-lbeta%NF-κB%Reverse transcriptase polymerase chain reaction%Immunohistochemistry
目的 观察肠瑞灌肠剂对直线加速器诱导的放射性直肠炎大鼠直肠黏膜IL-1β和NF-κB表达的影响,探讨其黏膜修复作用及抗炎机制.方法 利用直线加速器单次大剂量盆腔局部照射,建立放射性直肠炎大鼠模型.70只大鼠随机分为7组,三个治疗组分别给予高、中、低不同剂量肠瑞灌肠剂,西药对照组给予地塞米松+庆大霉素,中药对照组给予八味锡类散,模型组与空白组给予生理盐水,给药途径均保留灌肠.观察治疗1周后处死全部动物.分别用RT-PCR方法、免疫组织化学方法检测直肠黏膜IL-1β mRNA、NF-κB表达,进行统计学处理.结果 照射后直肠黏膜出现IL-1β mRNA及NF-κB表达,治疗组IL-1β mRNA表达及NF-κB表达显著减少,与模型组、中西药对照组比较差异有统计学意义(P<0.05).治疗高剂量组优于低剂量组.结论 肠瑞灌肠剂能下调放射性直肠炎大鼠直肠黏膜IL-1β mRNA及NF-κB表达,以炎症因子为药物靶点,减轻放射性直肠炎炎症反应,促进肠黏膜修复.
目的 觀察腸瑞灌腸劑對直線加速器誘導的放射性直腸炎大鼠直腸黏膜IL-1β和NF-κB錶達的影響,探討其黏膜脩複作用及抗炎機製.方法 利用直線加速器單次大劑量盆腔跼部照射,建立放射性直腸炎大鼠模型.70隻大鼠隨機分為7組,三箇治療組分彆給予高、中、低不同劑量腸瑞灌腸劑,西藥對照組給予地塞米鬆+慶大黴素,中藥對照組給予八味錫類散,模型組與空白組給予生理鹽水,給藥途徑均保留灌腸.觀察治療1週後處死全部動物.分彆用RT-PCR方法、免疫組織化學方法檢測直腸黏膜IL-1β mRNA、NF-κB錶達,進行統計學處理.結果 照射後直腸黏膜齣現IL-1β mRNA及NF-κB錶達,治療組IL-1β mRNA錶達及NF-κB錶達顯著減少,與模型組、中西藥對照組比較差異有統計學意義(P<0.05).治療高劑量組優于低劑量組.結論 腸瑞灌腸劑能下調放射性直腸炎大鼠直腸黏膜IL-1β mRNA及NF-κB錶達,以炎癥因子為藥物靶點,減輕放射性直腸炎炎癥反應,促進腸黏膜脩複.
목적 관찰장서관장제대직선가속기유도적방사성직장염대서직장점막IL-1β화NF-κB표체적영향,탐토기점막수복작용급항염궤제.방법 이용직선가속기단차대제량분강국부조사,건립방사성직장염대서모형.70지대서수궤분위7조,삼개치료조분별급여고、중、저불동제량장서관장제,서약대조조급여지새미송+경대매소,중약대조조급여팔미석류산,모형조여공백조급여생리염수,급약도경균보류관장.관찰치료1주후처사전부동물.분별용RT-PCR방법、면역조직화학방법검측직장점막IL-1β mRNA、NF-κB표체,진행통계학처리.결과 조사후직장점막출현IL-1β mRNA급NF-κB표체,치료조IL-1β mRNA표체급NF-κB표체현저감소,여모형조、중서약대조조비교차이유통계학의의(P<0.05).치료고제량조우우저제량조.결론 장서관장제능하조방사성직장염대서직장점막IL-1β mRNA급NF-κB표체,이염증인자위약물파점,감경방사성직장염염증반응,촉진장점막수복.
Objective To observe the effects of Changrui Enemas on IL-1β and NF-κB in the rat model of radiation-induced proctitis, and to explore the mechanism of its repairing mucous membrane and diminishing inflammation. Methods Radiation was given on the pelvis of rats through linear accelerator to establish the model of proctitis. 70 rats were divided into seven groups randomly. Small dose (0.4 g/ml), middle dose(0.8 g/ml) and large dose(1.6 g/ml) of Changrui Enemas by retention enema were given for 7 days, with the admixture liquids of Gentamyein and Dexamethasone, Xilei Powder was used as controls. Changes of IL-1β and NF-κB expression were investigated by semi-quantitative RT-PCR and IHC respectively. Results The expression of IL-1β and NF-κB were determined in the groups accepted radiation, but there had been a dramatic decline in the treatment groups(P<0.05). The high dose treatment group was superior to the low dose one on the whole. Conclusion Changrui Enemas can down-regulate the expression of inflammatory factor IL-1β and NF-κB on radiation induced proctitis rats, and reduce the inflammatory reaction, protect the rectal mucosa, accelerate healing up of the ulcer and relief the symptoms.
