中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
17期
1213-1215
,共3页
陈颖%佟晓红%孙成涓%张为远
陳穎%佟曉紅%孫成涓%張為遠
진영%동효홍%손성연%장위원
卵泡刺激素受体%酪氨酸羟化酶%单核苷酸多态性%子痫
卵泡刺激素受體%酪氨痠羥化酶%單覈苷痠多態性%子癇
란포자격소수체%락안산간화매%단핵감산다태성%자간
Follicle-stimulating hormone receptor%Tyrosine hydroxylase%Gene polymorphism%Pre-eclampsia
目的 探讨卵泡刺激素受体基因(FSHR)rs1394205多态性和酪氨酸羟化酶基因(TH)rs2070762多态性与子痫前期(PE)发病的关系.方法 采用TaqMan探针法,对105例PE患者和103例正常妊娠妇女的FSHR rs1394205多态性和TH rs2070762多态性位点进行基因型分析.结果 PE组FSHR rs1394205的基因型频率CC、CT、TT分别为23.8%、50.5%、25.7%,等位基因频率C、T分别为49.0%,51.0%.对照组基因型频率CC、CT、TT分别为23.3%、52.4%、24.3%,等位基因频率C、T分别为49.5%,50.5%,两组比较差异无统计学意义(P＞0.05);PE组TH基因rs2070762基因型频率CC、CT、TT分别为18.1%、56.2%、25.7%,等位基因频率C、T分别为46.2%,53.8%.对照组基因型频率CC、CT、TT分别为14.6%、42.7%、42.7%,等位基因频率C、T分别为35.9%、64.1%,两组比较PE组TT基因型频率和等位基因T频率明显低于对照组(P＜0.05).rs1394205和rs2070762均与PE疾病严重程度差异不明显.结论 TH基因rs2070762多态性(基因型,TT和等位基因T)可能是PE的保护性因素,FSHR基因rs1394205多态性与PE发病无关,2个SNP位点与PE的发病程度均无关.
目的 探討卵泡刺激素受體基因(FSHR)rs1394205多態性和酪氨痠羥化酶基因(TH)rs2070762多態性與子癇前期(PE)髮病的關繫.方法 採用TaqMan探針法,對105例PE患者和103例正常妊娠婦女的FSHR rs1394205多態性和TH rs2070762多態性位點進行基因型分析.結果 PE組FSHR rs1394205的基因型頻率CC、CT、TT分彆為23.8%、50.5%、25.7%,等位基因頻率C、T分彆為49.0%,51.0%.對照組基因型頻率CC、CT、TT分彆為23.3%、52.4%、24.3%,等位基因頻率C、T分彆為49.5%,50.5%,兩組比較差異無統計學意義(P＞0.05);PE組TH基因rs2070762基因型頻率CC、CT、TT分彆為18.1%、56.2%、25.7%,等位基因頻率C、T分彆為46.2%,53.8%.對照組基因型頻率CC、CT、TT分彆為14.6%、42.7%、42.7%,等位基因頻率C、T分彆為35.9%、64.1%,兩組比較PE組TT基因型頻率和等位基因T頻率明顯低于對照組(P＜0.05).rs1394205和rs2070762均與PE疾病嚴重程度差異不明顯.結論 TH基因rs2070762多態性(基因型,TT和等位基因T)可能是PE的保護性因素,FSHR基因rs1394205多態性與PE髮病無關,2箇SNP位點與PE的髮病程度均無關.
목적 탐토란포자격소수체기인(FSHR)rs1394205다태성화락안산간화매기인(TH)rs2070762다태성여자간전기(PE)발병적관계.방법 채용TaqMan탐침법,대105례PE환자화103례정상임신부녀적FSHR rs1394205다태성화TH rs2070762다태성위점진행기인형분석.결과 PE조FSHR rs1394205적기인형빈솔CC、CT、TT분별위23.8%、50.5%、25.7%,등위기인빈솔C、T분별위49.0%,51.0%.대조조기인형빈솔CC、CT、TT분별위23.3%、52.4%、24.3%,등위기인빈솔C、T분별위49.5%,50.5%,량조비교차이무통계학의의(P＞0.05);PE조TH기인rs2070762기인형빈솔CC、CT、TT분별위18.1%、56.2%、25.7%,등위기인빈솔C、T분별위46.2%,53.8%.대조조기인형빈솔CC、CT、TT분별위14.6%、42.7%、42.7%,등위기인빈솔C、T분별위35.9%、64.1%,량조비교PE조TT기인형빈솔화등위기인T빈솔명현저우대조조(P＜0.05).rs1394205화rs2070762균여PE질병엄중정도차이불명현.결론 TH기인rs2070762다태성(기인형,TT화등위기인T)가능시PE적보호성인소,FSHR기인rs1394205다태성여PE발병무관,2개SNP위점여PE적발병정도균무관.
Objective To analyze a potential association of gene polymorphisms of the folliclestimulating hormone receptor (FSHR) gene rs1394205 polymorphism and tyrosine hydroxylase (TH)rs2070762 polymorphism with pre-eclampsia. Methods The gene polymorphism of FSHR rs1394205 and TH rs2070762 were analyzed by real-time Quantitative polymerase chain reaction (TaqMan probe) in 105 patients with pre-eclampsia and 103 healthy pregnant subjects in Chinese Han population. Genotype and allele were assessed by direct counting methods. And the associations between 2 SNPs and pre-eclampsia were analyzed by chi-square test. Results The frequencies of the TH gene rs2070762 polymorphism CC, C/T and TT genotypes were 14. 6%, 42. 7% and 42. 7% in pre-eclampsia group and 18. 1%, 56. 2% and 25.7% in normal control group respectively. The allelic frequency of rs2070762 polymorphism in TH in preeclampsia patients was significantly lower ( P ＜ 0. 05 ) than that in normal pregnancies. There was a significant difference between two groups ( P ＜ 0. 05 ). But there was no significant difference between mild and severe pre-eclampsia groups ( P ＞ 0. 05 ). In contrast, no significant association was detected in the comparison of genotypes and allele of FSHR gene rs1394205 between pre-eclampsia patients and normal pregnancies. Conclusion The TH rs2070762 polymorphism was associated with the pathogenesis of preeclampsia. It suggests that the polymorphism in TH may be involved in the onset and development of preeclampsia.
