CAJ | 학술논문

目的研究单克隆和接种密度对永生化骨髓基质干细胞(hMSC-TERT)体内、外分化潜能的影响,寻找合理的体外培养模式.方法从亲代hMSC-TERT中,建立了30个单克隆细胞系,并以不同的接种密度建立了2个独立的细胞系.对各细胞系在体外向脂肪、骨、神经样细胞方向诱导分化,用免疫组化的方法测定细胞在SCID鼠体内的多向分化潜能.结果高接种密度细胞比单克隆和低接种密度细胞拥有更多的体外分化潜能,更好的保持了基质细胞特性.各单克隆细胞体内分化能力存在差异,甚至可以具有亲代细胞没有的分化能力.结论以高接种密度的方式培养多克隆原代细胞,能够保持细胞的原有特性.移植前,筛选特定的单克隆细胞,进行特定用途的移植.
목적 연구단극륭화접충밀도대영생화골수기질간세포(hMSC-TERT)체내、외분화잠능적영향,심조합리적체외배양모식.방법 종친대hMSC-TERT중,건립료30개단극륭세포계,병이불동적접충밀도건립료2개독립적세포계.대각세포계재체외향지방、골、신경양세포방향유도분화,용면역조화적방법측정세포재SCID서체내적다향분화잠능.결과 고접충밀도세포비단극륭화저접충밀도세포옹유경다적체외분화잠능,경호적보지료기질세포특성.각단극륭세포체내분화능력존재차이,심지가이구유친대세포몰유적분화능력.결론 이고접충밀도적방식배양다극륭원대세포,능구보지세포적원유특성.이식전,사선특정적단극륭세포,진행특정용도적이식.
Objective To determine whether monneionality or different cell seeding densities could influence the differentiation potential of immortalized human mesenchymal stem cells (hMSC-TERT) and to find an effective cultural method of hMSC-TERT in vitro. Methods From the parental hMSC-TERT cell line, we derived 30 monoclonal cell lines and two independent cell lines based on different plating densities during expansion in culture. Their adipocytic, osteogenic, neuronal differentiation potential in vitro and multidirectional differentiation potential in vivo were analyzed by immunohistochemistry for pathologic tissue markers. Results Monoclonal cell lines and the cell line derived at low seeding density had a lower differentiation potential in vitro than the cell line derived at higher cell seeding density. The differentiation potential of monoclonal hMSC-TERT cells were dissimilar. Some of monnelonal hMSC-TERT lines expressed epithelial differentiation potential in vivo while the parental hMSC-TERT cells line did not Conclusion Multiclonal hMSC-TERT cells cultured in high seeding density can keep the differentiation potential, cloning the hMSC-TERT cells before transplantation to find the special clones for special purpose of transplantation.