CAJ | 학술논문

目的探讨血管紧张素转换酶(angiotensin coverting enzyme,ACE)基因插/缺失(I/D)多态性与广西巴马长寿的关系.方法采用聚合酶链反应(PCR)、单链构象多态性分析(SSCP)与直接测序技术,研究百岁组(≥100岁)27例、90岁组(90～99岁)56例、老年组(60～89岁)114例老人的ACE基因I/D多态性,以79例60～70岁自然死亡人群为对照组.结果在ACE基因I/D多态基因型中,百岁组L/I、L/D、D/D型频率分别为37.04%、33.33%和29.63%,I、D等位基因频率分别为53.70和46.30%.百岁组D/D基因型频率和D型等位基因频率高于老年组(P<0.05)且明显高于对照组(P<0.01),但与90岁组比较,无论是I/I、I/D或D/D基因型,所占百分率均比较接近,差异无显著性(P>O.05).D/D基因型经SSCP证实全部相同,无差别.直接测序结果再次验证ACE(I/D)多态性判断准确.结论 ACE基因I/D多态性与长寿密切相关.长寿受多种因素影响.
목적 탐토혈관긴장소전환매(angiotensin coverting enzyme,ACE)기인삽/결실(I/D)다태성여엄서파마장수적관계.방법 채용취합매련반응(PCR)、단련구상다태성분석(SSCP)여직접측서기술,연구백세조(≥100세)27례、90세조(90～99세)56례、노년조(60～89세)114례노인적ACE기인I/D다태성,이79례60～70세자연사망인군위대조조.결과 재ACE기인I/D다태기인형중,백세조L/I、L/D、D/D형빈솔분별위37.04%、33.33%화29.63%,I、D등위기인빈솔분별위53.70화46.30%.백세조D/D기인형빈솔화D형등위기인빈솔고우노년조(P<0.05)차명현고우대조조(P<0.01),단여90세조비교,무론시I/I、I/D혹D/D기인형,소점백분솔균비교접근,차이무현저성(P>O.05).D/D기인형경SSCP증실전부상동,무차별.직접측서결과재차험증ACE(I/D)다태성판단준학.결론 ACE기인I/D다태성여장수밀절상관.장수수다충인소영향.
Objective To study the distribution of I/D polymorphisms of angiotensin coverting enzyme(ACE)gene and its relationship to longevity of Bama.Methods Polymerize chain reaction,single strand conformation polymorphism,and direct sequencing technique were used to test I/D polymorphisms of ACE gene in 27 centenarians,56persons aged 90～99,114 persons aged 60～89 and 79 cases of natural death aged 60～70 were used as controls.Resuits The frequencies of ACE genotypes I/I,I/D and D/D were 37.04%,33.33%,and 29.63% respectively in the centenarian group.The frequency rates of I and D alleles were 53.70% and 46.30%.The frequency rate of D/D genotype of the centenarian group was higher than that of the group aged 60～89(X~2=4.339,P<0.05),significantly higher than that of the group aged 60～70(X~2=7.145,P<0.01).However,it is not significantly different from that of the group aged 90～99(P>0.05).The frequency rate of D allele of the group of centenarian was higher than that of the group aged 60～70(X~2=4.466,P<0.05).The frequency rates of genotype D/D and D alleles were significantly higher in the centenarian group than that in the controls(both P<0.01).There is no difference between D/D genotype by single strand conformation polymorphism.Direct sequencing results reconfirmed the veracity of I/D polymorphisms of ACE gene.Conclusion ACE gene polymorphism is closely correlated with life span.In addition,longevity is the interaction of multiple factors.