CAJ | 학술논문

从长期富含枯枝落叶的土壤和造纸厂排污口污泥中分离筛选到一株能降解纤维素的放线菌,对其在以羧甲基纤维素为惟一碳源的产酶培养基上进行产酶条件研究,结果表明,该菌产酶较合适的温度是28℃,培养基起始pH值是7.0,接种培养24 h的种子液产酶量较高,以2%的接种量接种酶活较高,其在优化条件下的酶活可达到44.76 U/mL.
종장기부함고지락협적토양화조지엄배오구오니중분리사선도일주능강해섬유소적방선균,대기재이최갑기섬유소위유일탄원적산매배양기상진행산매조건연구,결과표명,해균산매교합괄적온도시28℃,배양기기시pH치시7.0,접충배양24 h적충자액산매량교고,이2%적접충량접충매활교고,기재우화조건하적매활가체도44.76 U/mL.
A Cellulose-decomposing actinomyces strain was isolated from the rich humus soil and the sludge in limberhole of the paper mill.Carboxymethyl cellulose as the sole carbon source in the media was used in the study of enzyme-producing condition.The results showed that the optimum enzyme-producing conditions were at 28℃ with medium primary pH 7.0,and inoculated with 2%inoculum which had been cultivated for 24 hours.Its enzyme activity could reach 44.76U/mL under the optimum conditions.