CAJ | 학술논문

目的分析系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)蛋白质表达谱的变化.方法分别抽取SLE患者及健康对照外周血,分离单个核细胞,抽提总蛋白,进行双向电泳、电泳胶银染显色,用ImageMaster 2D Platinum 5.0软件对获得的蛋白图谱进行分析,寻找差异表达的蛋白质.利用基质辅助激光解析电离飞行时间质谱鉴定差异蛋白点.结果对照组凝胶蛋白点匹配率为(71±4)%,患者组匹配率为(72±4)%.对照组凝胶共检出蛋白点(791±17)个,患者组检出(781±17)个.有11个蛋白点在SLE患者组表达上调,9个表达下调,质谱分析共鉴定5个蛋白.以前的研究显示,我们鉴定的部分蛋白在SLE的发病机制中起潜在的作用.结论 SLE患者外周血单个核细胞蛋白质表达发生了明显改变,为我们从淋巴细胞蛋白质谱变化的整体角度上阐明SLE发生的分子机制及免疫调控通路奠定了基础.
목적 분석계통성홍반랑창(SLE)환자외주혈단개핵세포(PBMCs)단백질표체보적변화.방법 분별추취SLE환자급건강대조외주혈,분리단개핵세포,추제총단백,진행쌍향전영、전영효은염현색,용ImageMaster 2D Platinum 5.0연건대획득적단백도보진행분석,심조차이표체적단백질.이용기질보조격광해석전리비행시간질보감정차이단백점.결과 대조조응효단백점필배솔위(71±4)%,환자조필배솔위(72±4)%.대조조응효공검출단백점(791±17)개,환자조검출(781±17)개.유11개단백점재SLE환자조표체상조,9개표체하조,질보분석공감정5개단백.이전적연구현시,아문감정적부분단백재SLE적발병궤제중기잠재적작용.결론 SLE환자외주혈단개핵세포단백질표체발생료명현개변,위아문종림파세포단백질보변화적정체각도상천명SLE발생적분자궤제급면역조공통로전정료기출.
Objective To analyze the changes in the protein expression profile of peripheral blood mononuclear cells in systemic lupus erythematosus patients. Methods Peripheral blood was obtained from SLE patients and healthy controls, then mononuclear cells were isolated and the total protein was extracted by one-step method. Two-dimensional gel electrophoresis was performed and then stained with silver. Protein maps were analyzed and differentially expressed protein spots were detected using ImageMaster 2D Platinum 5.0 software. Results Match rates of (71±4)% and (72±4)% was obtained from gels from controls and pati-ents respectively. 791±17 spots were detected from control gels and 781±17 from patient gels. Eleven protein spots were up-regulated and 9 were down-regulated in SLE patients. Five proteins were identified by MS analysis, some of which had previously been shown to play a potential role in the pathogenesis of SLE. Conclusion There are significant changes in the protein expression of peripheral blood mononuclear cells in systemic lupus erythematosus patients. This study could be used as a preliminary work for better understanding of the pathogenesis and immune regulation pathways of SLE from an integrated lymphocyte protein profile perspective.