中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2009年
1期
39-42
,共4页
许焕丽%稻垣善则%王凤山%张克明%国土典宏%唐伟
許煥麗%稻垣善則%王鳳山%張剋明%國土典宏%唐偉
허환려%도원선칙%왕봉산%장극명%국토전굉%당위
癌%肝细胞%KL-6黏蛋白%胆管细胞癌%转移性肝癌
癌%肝細胞%KL-6黏蛋白%膽管細胞癌%轉移性肝癌
암%간세포%KL-6점단백%담관세포암%전이성간암
Carcinoma,hepatoeellular%KL-6 mucin%cholangiocarcinoma%Metastatic liver cancer
目的 检测KL-6黏蛋自在不同类型肝癌患者癌组织和血清中的表达水平,并探讨其作为肿瘤标志物在临床诊断中应用的价值.方法 采用免疫组织化学方法检测81例肝细胞癌(HCC)、21例胆管细胞癌(CC)、12例混合型肝细胞-胆管细胞癌(HCC-CC)和56例原发性结直肠癌肝转移(MLC)患者癌组织中KL-6黏蛋白的表达水平.用ELISA法测定其中34例HCC患者、8例CC患者、30例MLC患者和19例健康自愿者血清中KL-6黏蛋白的表达水平,并用t检验分析其结果.结果 免疫组织化学分析结果表明,所有的CC患者和HCC-CC患者中,CC组织(12/12,100%)KL-6黏蛋白均呈阳性染色,而MLC患者的癌组织(56/56,100%)显示数个癌细胞或部分癌组织呈阳性染色.另外,在HCC和HCC-CC患者的HCC组织以及所有的非癌组织中KL-6黏蛋白没有发现阳性染色.CC患者的血清KL-6黏蛋白表达水平比健康自愿者、HCC患者和MLC患者高(t=5.58,5.34,4.00,P＜0.01).MLC患者和健康自愿者的血清KL-6黏蛋白表达水平比较,差异有统计学意义(t=2.77,P＜0.01).健康自愿者和HCC患者,以及MLC患者和HCC患者之间的血清KL-6黏蛋白表达水平比较,差异无统计学意义(t=2.03,1.89,P＞0.05).结论 KL-6黏蛋白在CC患者癌组织和血清中的表达水平均比HCC患者、MLC患者和健康自愿者高,可成为CC患者的特异性肿瘤标志物.
目的 檢測KL-6黏蛋自在不同類型肝癌患者癌組織和血清中的錶達水平,併探討其作為腫瘤標誌物在臨床診斷中應用的價值.方法 採用免疫組織化學方法檢測81例肝細胞癌(HCC)、21例膽管細胞癌(CC)、12例混閤型肝細胞-膽管細胞癌(HCC-CC)和56例原髮性結直腸癌肝轉移(MLC)患者癌組織中KL-6黏蛋白的錶達水平.用ELISA法測定其中34例HCC患者、8例CC患者、30例MLC患者和19例健康自願者血清中KL-6黏蛋白的錶達水平,併用t檢驗分析其結果.結果 免疫組織化學分析結果錶明,所有的CC患者和HCC-CC患者中,CC組織(12/12,100%)KL-6黏蛋白均呈暘性染色,而MLC患者的癌組織(56/56,100%)顯示數箇癌細胞或部分癌組織呈暘性染色.另外,在HCC和HCC-CC患者的HCC組織以及所有的非癌組織中KL-6黏蛋白沒有髮現暘性染色.CC患者的血清KL-6黏蛋白錶達水平比健康自願者、HCC患者和MLC患者高(t=5.58,5.34,4.00,P＜0.01).MLC患者和健康自願者的血清KL-6黏蛋白錶達水平比較,差異有統計學意義(t=2.77,P＜0.01).健康自願者和HCC患者,以及MLC患者和HCC患者之間的血清KL-6黏蛋白錶達水平比較,差異無統計學意義(t=2.03,1.89,P＞0.05).結論 KL-6黏蛋白在CC患者癌組織和血清中的錶達水平均比HCC患者、MLC患者和健康自願者高,可成為CC患者的特異性腫瘤標誌物.
목적 검측KL-6점단자재불동류형간암환자암조직화혈청중적표체수평,병탐토기작위종류표지물재림상진단중응용적개치.방법 채용면역조직화학방법검측81례간세포암(HCC)、21례담관세포암(CC)、12례혼합형간세포-담관세포암(HCC-CC)화56례원발성결직장암간전이(MLC)환자암조직중KL-6점단백적표체수평.용ELISA법측정기중34례HCC환자、8례CC환자、30례MLC환자화19례건강자원자혈청중KL-6점단백적표체수평,병용t검험분석기결과.결과 면역조직화학분석결과표명,소유적CC환자화HCC-CC환자중,CC조직(12/12,100%)KL-6점단백균정양성염색,이MLC환자적암조직(56/56,100%)현시수개암세포혹부분암조직정양성염색.령외,재HCC화HCC-CC환자적HCC조직이급소유적비암조직중KL-6점단백몰유발현양성염색.CC환자적혈청KL-6점단백표체수평비건강자원자、HCC환자화MLC환자고(t=5.58,5.34,4.00,P＜0.01).MLC환자화건강자원자적혈청KL-6점단백표체수평비교,차이유통계학의의(t=2.77,P＜0.01).건강자원자화HCC환자,이급MLC환자화HCC환자지간적혈청KL-6점단백표체수평비교,차이무통계학의의(t=2.03,1.89,P＞0.05).결론 KL-6점단백재CC환자암조직화혈청중적표체수평균비HCC환자、MLC환자화건강자원자고,가성위CC환자적특이성종류표지물.
Objective To detect the expression of KL-6 mucin in the tissue and serum of hepatoma in different hepatoma patients,and to investigate the value of KL-6 mucin as a tumor marker in the diagnosis of hepatoma.Methods The expression of KL-6 mucin in the hepatoma tissues of 81 patients with hepatocellular carcinoma(HCC),21 patients with cholangiocarcinoma(CC),12 patients with combined hepatocellular and cholangiocarcinoma(HCC-CC)and 56 patients with metastatic liver cancer(MLC)was detected by immunohistochemical analysis.The expression of KL-6 mucin in the serums of 34 HCC patients,8 CC patients,30 MLC patients and 19 healthy individuals was detected by enzyme-linked immunosorbent assay,and all the data were analyzed by t test.Results Expression of KL-6 mucin was detected in the cholangiocarcinoma tissues in all CCand HCC-CC patients.In several hepatoma cells and partial hepatoma tissues of patients with MLC,the expression of KL-6 mucin was detected.No expression of KL-6 mucin was detected in the hepatocellular carcinoma tissuesand non-cancerous tissues of patients with HCC or HCC-CC.The serum levels of KL-6 mucin expression in CC patients were signifcantly higher than those in healthy individuals,HCC and MLC patients(t=5.58,5.34,4.00.P＜0.01).The difference of the serum levels of KL-6 mucin expression between MLC patients and healthy individuals had statistical significance(t=2.77,P＜0.01).However,no significant difference in serum levels of KL-6 mucin expression was found between HCC patients and healthy individuals and between HCC patients and MLC patients(t=2.03,1.89,P＞0.05).Conclusion The expression of KL-6 inucin in CC patients is significantly higher than in patients with other types of hepatoma in both tissue and serum levels.Thus,KL-6 may be a usetul new tmnor marker for distinguishing CC from other types of hepatoma.
