CAJ | 학술논문

目的建立Ⅱ型胶原诱导性大鼠关节炎模型(CIA),结合病理改变研究磁共振成像(MRI)检测对早期类风湿关节炎的诊断价值.方法用牛Ⅱ型胶原和不完全弗氏佐剂联合皮内注射免疫Wistsr大鼠,制作关节炎模型;免疫后不同时期分别采用普通X线摄片、1.5T磁共振仪进行双踝关节平扫加增强扫描以及病理组织学等方法进行大鼠踝关节病理学特征分析.结果 CIA实验组大鼠免疫14 d后,其关节指数(AI)显著增加,28 d AI增加达到最高(3.6±1.0),抗Ⅱ型胶原抗体水平较对照组明显高(P<0.001).根据病理学分析CIA成模率为93.3%.MRI平扫加增强显示有12只出现异常征象,敏感性85.7%,特异性100%,与病理诊断呈正相关(r=0.5345,P<0.05).X线有4只出现关节软组织肿胀,MRI较X线检出率高(P=0.008).结论 MRI对早期关节滑膜炎、滑膜增生、血管翳等病理学的诊断生成方面有明显优势.
목적 건립Ⅱ형효원유도성대서관절염모형(CIA),결합병리개변연구자공진성상(MRI)검측대조기류풍습관절염적진단개치.방법 용우Ⅱ형효원화불완전불씨좌제연합피내주사면역Wistsr대서,제작관절염모형;면역후불동시기분별채용보통X선섭편、1.5T자공진의진행쌍과관절평소가증강소묘이급병리조직학등방법 진행대서과관절병이학특정분석.결과 CIA실험조대서면역14 d후,기관절지수(AI)현저증가,28 d AI증가체도최고(3.6±1.0),항Ⅱ형효원항체수평교대조조명현고(P<0.001).근거병이학분석CIA성모솔위93.3%.MRI평소가증강현시유12지출현이상정상,민감성85.7%,특이성100%,여병리진단정정상관(r=0.5345,P<0.05).X선유4지출현관절연조직종창,MRI교X선검출솔고(P=0.008).결론 MRI대조기관절활막염、활막증생、혈관예등병이학적진단생성방면유명현우세.
Objective To establish the rat model of collagen-induced arthritis and study the diagnostic value of MRI for early RA by comparing to pathological changes. Methods Thirty Wistar rats were randomly divided into normal groups and experimental groups.Fifteen rats of the experimental groups were immunized with type Ⅱ collagen and Freund's Adjuvant Incomplete. At the scheduled days, the selected rats of both experimental and normal groups underwent X-ray, 1.5T MR scan plus enhancement and histological analysis. Results Arthritis Index increased on the 14th day after immunization and reached the peak on the 28th day in the experimental group, the positivity of Anti-CⅡ antibody was significantly different from the normal group.The experiemental model was established in 93.33% of all rats. The enhanced MRI showed that 12 (12/15) rats presented with abnormal signs . The sensitivity of MRI was 85.71% and the specificity was 100%. There was no significant correlation could be found between MRI and histological changes X-ray revealed soft tissue swollen in 4 (4/15) rats, which showed that MRI had higher sensitivity in detecting abnormal signs of arthritis than X-ray. Conclusion MRI may be used in the early diagnosis of early RA.