CAJ | 학술논문

目的评价灵芝孢子对人卵巢癌顺铂耐药细胞株(A2780CP)耐药性的影响.方法人卵巢癌顺铂耐药细胞株A2780CP随机分为对照组和实验组,分别于培养液、含0.5 mg/ml灵芝孢子的培养液孵育48 h,采用WST-1法确定细胞顺铂耐药倍数;采用Western blot法测定细胞丝氨酸/苏氨酸蛋白激酶(Akt)、Bcl-2及p53蛋白的表达.结果与对照组相比,实验组耐药倍数明显降低,Akt、Bcl-2蛋白表达下调,而p53蛋白表达上调(P<0.05).绪论灵芝孢子可有效降低人卵巢癌细胞的顺铂耐药性,其机制与下调Akt及Bcl-2蛋白、上调p53蛋白表达有关.
목적 평개령지포자대인란소암순박내약세포주(A2780CP)내약성적영향.방법 인란소암순박내약세포주A2780CP수궤분위대조조화실험조,분별우배양액、함0.5 mg/ml령지포자적배양액부육48 h,채용WST-1법학정세포순박내약배수;채용Western blot법측정세포사안산/소안산단백격매(Akt)、Bcl-2급p53단백적표체.결과 여대조조상비,실험조내약배수명현강저,Akt、Bcl-2단백표체하조,이p53단백표체상조(P<0.05).서론령지포자가유효강저인란소암세포적순박내약성,기궤제여하조Akt급Bcl-2단백、상조p53단백표체유관.
Objective To evaluate the effect of Ganoderma lucidum on drug resistance of the cisplantin-resistant ovarian cancer cell line(A2780CP).Methods The A2780CP cells were randomly divided into control group and test group.The cells in control group and test group were incubated in culture media alone and in culture media containing Ganoderma lucidum 0.5 mg/ml for 48 h respectively.The resistance index(RI) of A2780CP was determined by WST-1 assay.The expression of Akt,Bcl-2 and p53 protein was measured by Western blot.Results The RI was significantly deeressed,the expression of Akt and Bcl-2 protein was down-regulated,while p53 protein expression was up-regulated in test group as compared with control group(P<0.05).Conclusion Ganoderma lueidum can reduce the resistance of A2780 CP cells to cisplantin by down-regulating the expression of Akt and Bcl-2 and up-regulating p53 expression in A2780CP cells.