CAJ | 학술논문

目的探讨阿维A酸对人皮肤鳞癌细胞系SCC13细胞的生长抑制、凋亡的诱导作用以及对caspases蛋白的影响.方法以体外培养的人皮肤鳞癌细胞系SCC13细胞为研究对象,采用MTT法观察5种浓度(5×10-7、1×10-6、5×10-6、1×10-5、5×10-5moL/L)阿维A酸对SCC13细胞的生长抑制作用.ELISA检测凋亡的诱导情况.流式细胞仪检测细胞周期.Western blot分析caspase-8和caspase-9的蛋白表达.结果阿维A酸能显著抑制人皮肤鳞癌细胞的生长(第1、3、5天F值分别为83.64、96.34和123.17)、诱导其凋亡(第1、3、5天F值分别为74.45、107.37和64.28),具有浓度及时间依赖性(P均<0.05).阿维A酸能将细胞周期阻滞于G1期,作用SCC13细胞72 h后,G1期细胞比率明显上升为77.05%,而对照组仅为63.55%.阿维A酸作用SCC13细胞12 h后开始活化caspase-8蛋白,24 h后开始活化caspase-9蛋白,并具有时间依赖性(P均<0.01).结论阿维A酸能抑制皮肤鳞癌细胞生长并诱导凋亡,这一效应可能由Fas死亡受体和线粒体途径共同介导.
목적 탐토아유A산대인피부린암세포계SCC13세포적생장억제、조망적유도작용이급대caspases단백적영향.방법 이체외배양적인피부린암세포계SCC13세포위연구대상,채용MTT법관찰5충농도(5×10-7、1×10-6、5×10-6、1×10-5、5×10-5moL/L)아유A산대SCC13세포적생장억제작용.ELISA검측조망적유도정황.류식세포의검측세포주기.Western blot분석caspase-8화caspase-9적단백표체.결과 아유A산능현저억제인피부린암세포적생장(제1、3、5천F치분별위83.64、96.34화123.17)、유도기조망(제1、3、5천F치분별위74.45、107.37화64.28),구유농도급시간의뢰성(P균<0.05).아유A산능장세포주기조체우G1기,작용SCC13세포72 h후,G1기세포비솔명현상승위77.05%,이대조조부위63.55%.아유A산작용SCC13세포12 h후개시활화caspase-8단백,24 h후개시활화caspase-9단백,병구유시간의뢰성(P균<0.01).결론 아유A산능억제피부린암세포생장병유도조망,저일효응가능유Fas사망수체화선립체도경공동개도.
Objective To investigate the inducing effect of acitrotin on the growth and apoptosis of human cutaneous squamous cell carcinoma cell line SCC13 and on caspases expression.Methods Human cutaneous squa-mous cell carcinoma cell line SCC13 was treated with five different concentrations of acitrefin [5×10-7,1×10-6,5×10-6,1×10-5,5×10-5mol/L].Cell proliferation was evaluated by MTT assay.Apoptosis was assessed by en-zyme-linked immunosorbent assay.The cell cycle was assessed by flow cytometry.The protein expressions of caspase-8 and caspase-9 were examined with Western blot.Results Acitretin inhibited the growth ( F = 83.64,96.34 and 123.17, respectively on the first, third and fifth day)and induced the apoptosis of SCC13 cells(F=74.45,107.37,and 64.28, respectively on the first, third and fifth day) in a dose- and time-dependent manner(P<0.05).Acitretin altered cell cycle distribution of SCC13 cells as compared with controls, the G1-phase population increased by 77.66% 72 hours after acitretin treatment, while the control increased only by 63.55%.An active fragment of caspase-8 occurred following 12 hours treatment of acitretin on SCC13 cells, whereas the caspase-9 active fragment occurred 24 hours after acitretin treatment, which increased time-dependently (P<0.01).Conclusion Acitretin plays an important role on the growth inhibition and apoptosis of cutaneous squamous cell carcinoma cells, which may be affected through both Fas receptor way and mitochondria way.