中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2008年
3期
272-274
,共3页
秦汉%彭晓红%程江霞%缪希莉
秦漢%彭曉紅%程江霞%繆希莉
진한%팽효홍%정강하%무희리
哌啶类%心肌再灌注损伤%缺血预处理心肌
哌啶類%心肌再灌註損傷%缺血預處理心肌
고정류%심기재관주손상%결혈예처리심기
Piperidines%Myocardial reperfusion injury%Ischemic preconditioning,myocardial
目的 探讨瑞芬太尼对大鼠心肌缺血再灌注时血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)及白细胞介素-6(IL-6)浓度的影响.方法 雄性SD大鼠32只,体重200～250 g,随机分为假手术组(S组)、缺血再灌注组(I/R组)、缺血预处理组(IP组)和瑞芬太尼组(R组),每组8只.I/R组阻断冠状动脉左前降支(LAD)30 min,开放120 min;IP组阻断LAD 5 min,开放10 min,阻断30 min,开放120 min;R组静脉输注瑞芬太尼0.5 μg·kg-1·min-1 20 min,阻断LAD 30 min,开放120 min,此期间静脉输注100 μg/L瑞芬太尼,速率0.5 μg·kg-1·min-1.分别于再灌注120 min取颈内静脉血样,并取左心室心肌组织.ELISA法测定血清TNF-α、IL-Iβ及IL-6浓度,电镜下观察心肌细胞超微结构.结果 与S组比较,其余3组血清TNF-α、IL-1β和IL-6浓度升高(P＜0.01);与I/R组比较,IP组和R组血清TNF-α、IL-1β和IL-6浓度均降低(P＜0.01);与IP组比较,R组血清TNF-α和IL-1β浓度降低(P＜0.05).R组和IP组心肌细胞损伤程度轻于I/R组.结论 瑞芬太尼可通过降低血清TNF-α、IL-1β和IL-6浓度减轻大鼠心肌缺血再灌注损伤.
目的 探討瑞芬太尼對大鼠心肌缺血再灌註時血清腫瘤壞死因子-α(TNF-α)、白細胞介素-1β(IL-1β)及白細胞介素-6(IL-6)濃度的影響.方法 雄性SD大鼠32隻,體重200～250 g,隨機分為假手術組(S組)、缺血再灌註組(I/R組)、缺血預處理組(IP組)和瑞芬太尼組(R組),每組8隻.I/R組阻斷冠狀動脈左前降支(LAD)30 min,開放120 min;IP組阻斷LAD 5 min,開放10 min,阻斷30 min,開放120 min;R組靜脈輸註瑞芬太尼0.5 μg·kg-1·min-1 20 min,阻斷LAD 30 min,開放120 min,此期間靜脈輸註100 μg/L瑞芬太尼,速率0.5 μg·kg-1·min-1.分彆于再灌註120 min取頸內靜脈血樣,併取左心室心肌組織.ELISA法測定血清TNF-α、IL-Iβ及IL-6濃度,電鏡下觀察心肌細胞超微結構.結果 與S組比較,其餘3組血清TNF-α、IL-1β和IL-6濃度升高(P＜0.01);與I/R組比較,IP組和R組血清TNF-α、IL-1β和IL-6濃度均降低(P＜0.01);與IP組比較,R組血清TNF-α和IL-1β濃度降低(P＜0.05).R組和IP組心肌細胞損傷程度輕于I/R組.結論 瑞芬太尼可通過降低血清TNF-α、IL-1β和IL-6濃度減輕大鼠心肌缺血再灌註損傷.
목적 탐토서분태니대대서심기결혈재관주시혈청종류배사인자-α(TNF-α)、백세포개소-1β(IL-1β)급백세포개소-6(IL-6)농도적영향.방법 웅성SD대서32지,체중200～250 g,수궤분위가수술조(S조)、결혈재관주조(I/R조)、결혈예처리조(IP조)화서분태니조(R조),매조8지.I/R조조단관상동맥좌전강지(LAD)30 min,개방120 min;IP조조단LAD 5 min,개방10 min,조단30 min,개방120 min;R조정맥수주서분태니0.5 μg·kg-1·min-1 20 min,조단LAD 30 min,개방120 min,차기간정맥수주100 μg/L서분태니,속솔0.5 μg·kg-1·min-1.분별우재관주120 min취경내정맥혈양,병취좌심실심기조직.ELISA법측정혈청TNF-α、IL-Iβ급IL-6농도,전경하관찰심기세포초미결구.결과 여S조비교,기여3조혈청TNF-α、IL-1β화IL-6농도승고(P＜0.01);여I/R조비교,IP조화R조혈청TNF-α、IL-1β화IL-6농도균강저(P＜0.01);여IP조비교,R조혈청TNF-α화IL-1β농도강저(P＜0.05).R조화IP조심기세포손상정도경우I/R조.결론 서분태니가통과강저혈청TNF-α、IL-1β화IL-6농도감경대서심기결혈재관주손상.
Objective To investigate the effects of remifentanil on the serum concentrations of TNF-α,IL-1β and IL-6 following myocardial ischemia-reperfusion(I/R)injury in rats.Methods Thirty-two male SD rats weighing 200-250 g were randomly divided into 4 groups(n=8 each):group S sham operation;group I/R;group IP ishemic preconditioning and group R remifentanil + I/R.In group I/R,anterior descending branch of left coronary artery(LAD)was accluded for 30 min,followed by 120 min reperfusion.In group IP,LAD was occluded for 5 min,followed by 10 min reperfusion,and then occluded for 30 min,followed by 120 min reperfusion.After this process.Blood samples from internal jugular vein and left ventricle myocardium tissue were taken at 120 min after the start of reperfusion for the determination of the serum concentrations of TNF-α,IL-1β and IL-6 by ELISA,and the myocardial histopathological changes were observed with electron microscopy.Results The serum concentrations of TNF-α,IL-1β and IL-6 were significantly higher in the other 3 groups than in group S,while significantly lower in group IP and group R than in group I/R(P＜0.01).The serum concentrations of TNF-α and IL-1β were significantly lower in group R than in group IP(P＜0.05).Myocardium injury was slighter in group R and group IP than in group I/R(P＜0.01).Conclusion Remifentanil can reduce the myocardial ischemiareperfusion injury through decreasing the serum concentrations of TNF-α,IL-1β and IL-6.
