CAJ | 학술논문

目的研究组织张力的变化对胶原排列结构的影响,探讨组织结构和力学性质的关系. 方法通过倒置相差显微镜、HE染色及透射电镜观察三种不同张力的胶原凝胶中成纤维细胞(fibroblast,Fb)的细胞形态、走行方向及胶原的排列结构;免疫荧光检测Fb中α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达及排布. 结果倒置相差显微镜、HE染色及透射电镜观察发现,单层培养基质(monolayer gels,MG)及锚着基质(anchored gels,AG)张力高,张力分布各向异性,Fb呈长梭形,极性明显,胶原纤维沿细胞长轴平行排列,尤以MG更为明显;游离基质(floating gels,FG)张力低,张力分布各向同性,Fb为多角形或星形,胶原纤维呈网状排列;免疫荧光检测显示,MG及锚着基质中Fb表达大量沿细胞长轴排列的α-SMA,尤以MG明显,游离基质中Fb少有α-SMA表达.MG及锚着基质中Fb细胞形态、走行方向、α-SMA的表达及胶原的排列结构近似于肉芽组织,游离基质则近似于正常皮肤组织或重塑完成后的创面组织. 结论皮组织的组织结构与组织的力学性质是相对应的,组织的排列结构是组织力学性质的体现,两者是不可截然分开的.
목적 연구조직장력적변화대효원배렬결구적영향,탐토조직결구화역학성질적관계. 방법 통과도치상차현미경、HE염색급투사전경관찰삼충불동장력적효원응효중성섬유세포(fibroblast,Fb)적세포형태、주행방향급효원적배렬결구;면역형광검측Fb중α평활기기동단백(α-smooth muscle actin,α-SMA)적표체급배포. 결과 도치상차현미경、HE염색급투사전경관찰발현,단층배양기질(monolayer gels,MG)급묘착기질(anchored gels,AG)장력고,장력분포각향이성,Fb정장사형,겁성명현,효원섬유연세포장축평행배렬,우이MG경위명현;유리기질(floating gels,FG)장력저,장력분포각향동성,Fb위다각형혹성형,효원섬유정망상배렬;면역형광검측현시,MG급묘착기질중Fb표체대량연세포장축배렬적α-SMA,우이MG명현,유리기질중Fb소유α-SMA표체.MG급묘착기질중Fb세포형태、주행방향、α-SMA적표체급효원적배렬결구근사우육아조직,유리기질칙근사우정상피부조직혹중소완성후적창면조직. 결론 피조직적조직결구여조직적역학성질시상대응적,조직적배렬결구시조직역학성질적체현,량자시불가절연분개적.
Objective To study the effect of mechanical tension on collagen arrangement and illustrate the relationship between tissue architecture and tension properties. Methods Cell morphologies, orientation and collagen arrangement of fibroblasts cultured in three different types of collagen gels with variation of mechanical tension were observed by phase contrast photomicrographs, light microscopy and transmission electron microscopy. Expression and distribution of a-smooth muscle actin (α-SMA) were investigated by immunohistochemistry. Results Phase contrast photomicrographs, light microscopy and transmission electron microscopy showed high level of tension distributed anisotropically in the monolayer gels and the anchored collagen gels, with bipolar shape of the fibroblasts, obvious polarity, arrangement of exogenous collagen fibres parallel to the long axis of the fibroblasts, especially prominent in monolayer gels. Low level of tension distributed isotropically was observed in floating collagen gels, with stellate morphology and arrangement of exogenous collagen fibres in a reticular array. Immunofluorescence showed that fibroblasts expressed high level of α-SMA protein distributed along the long axis of fibroblasts in the monolayer gels and the anchored collagen gels, especially in former ones. In contrast, few expression of α-SMA protein was found in floating collagen gels. Cell morphologies and orientation, expression and distribution of α-SMA as well as collagen arrangement of fibroblasts in the monolayer gels and the anchored collagen gels were similar to those in granulation tissue, whereas floating collagen gels resembled normal dermis or remodelled tissues. Conclusions Tissue architecture or morphology of the dermis are corresponding to tension proporties. Different tissue architectures are closely correlated with particular tension proporties.