CAJ | 학술논문

对转基因抗虫水稻中Bt蛋白表达量进行研究。[方法]应用酶联免疫吸附测定法（ELJsA）定量检测转基因抗虫水稻生同生长期不同部位的Bt蛋自表达量。[结果]转基因水稻灌浆期不同组织中Bt蛋白表达绝对含量的高低顺序为：叶片〉未成熟种子及颖壳〉根〉茎杆；在水稻不同的生长发育（分蘖期、抽穗期和灌浆期）阶段，转基因Bt水稻中Bt蛋白的含量有一些变化；一般在水稻生长后期Bt蛋白的浓度有所下降，但幅度不大，对其抗性不会造成太大影响。[结论]该试验对田间害虫的防治以及转基因水稻的育种都具有重要意义。
대전기인항충수도중Bt단백표체량진행연구。[방법]응용매련면역흡부측정법（ELJsA）정량검측전기인항충수도생동생장기불동부위적Bt단자표체량。[결과]전기인수도관장기불동조직중Bt단백표체절대함량적고저순서위：협편〉미성숙충자급영각〉근〉경간；재수도불동적생장발육（분얼기、추수기화관장기）계단，전기인Bt수도중Bt단백적함량유일사변화；일반재수도생장후기Bt단백적농도유소하강，단폭도불대，대기항성불회조성태대영향。[결론]해시험대전간해충적방치이급전기인수도적육충도구유중요의의。
[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.