四川理工学院学报：自然科学版
四川理工學院學報：自然科學版
사천리공학원학보：자연과학판
Journal of Sichuan University of Science & Engineering:Natural Science Editton
2012年
1期
14-18
,共5页
左勇%李杨%任永利%鞠帅%谢晖%祁峰
左勇%李楊%任永利%鞠帥%謝暉%祁峰
좌용%리양%임영리%국수%사휘%기봉
工色糖多孢茵%诱变%正交实验%发酵
工色糖多孢茵%誘變%正交實驗%髮酵
공색당다포인%유변%정교실험%발효
Saccharopolyspora erythraea%mutagenesis%orthogonal test%fermentation
以红色糖多孢菌08L作为出发菌株进行诱变，选育高产菌株。结果表明，诱变得到的UL5菌株发酵水平比出发菌株提高12．6％。再通过培养基优化，其组成为淀粉3％，糊精3％，葡萄糖2％，黄豆饼粉3％，玉米浆1％，硫酸铵0．15％，碳酸钙0．6％，磷酸二氢钾0．02％的条件下，UL5菌株在诱变的基础上，发酵水平再提高15．2％。
以紅色糖多孢菌08L作為齣髮菌株進行誘變，選育高產菌株。結果錶明，誘變得到的UL5菌株髮酵水平比齣髮菌株提高12．6％。再通過培養基優化，其組成為澱粉3％，糊精3％，葡萄糖2％，黃豆餅粉3％，玉米漿1％，硫痠銨0．15％，碳痠鈣0．6％，燐痠二氫鉀0．02％的條件下，UL5菌株在誘變的基礎上，髮酵水平再提高15．2％。
이홍색당다포균08L작위출발균주진행유변，선육고산균주。결과표명，유변득도적UL5균주발효수평비출발균주제고12．6％。재통과배양기우화，기조성위정분3％，호정3％，포도당2％，황두병분3％，옥미장1％，류산안0．15％，탄산개0．6％，린산이경갑0．02％적조건하，UL5균주재유변적기출상，발효수평재제고15．2％。
The Saccharopolyspora erythraea 08L is used as the starting strain. Processed by ultraviolet mutagenesis, the fermentation product of the mutated strains was increased by 12.6%. In addition, under the optimized culture medium which consisted of starch 3%, dextrin 3%, glucose 2% , soybean meal 3%, corn steep liquor 1%, （ NH4 ）2SO4 0.15%, CaCOz0. 6%, KH2PO40.02%, the fementation yield of UL5 strains was increased by 15.2%.
