激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2012年
1期
72-73
,共2页
血管性痴呆%粉防己碱%S100B%钙离子%双侧颈总动脉结扎
血管性癡呆%粉防己堿%S100B%鈣離子%雙側頸總動脈結扎
혈관성치태%분방기감%S100B%개리자%쌍측경총동맥결찰
vascular dementia%tetrandrine%S100B%calcium%permanent bilateral common carotid artery ligation
目的：探讨粉防己碱（Tet）对血管性痴呆（VaD）大鼠齿状回S1000B蛋白表达的影响以及在血管性痴呆发病中的作用。方法：运用双侧颈总动脉永久性结扎（2-VO法）建立VaD大鼠模型,Nissl染色观察海马齿状回（DG）神经元内的尼氏小体的变化,免疫荧光观察海马DG区S100B的表达情况,F1uo-3/AM探针负载后检测各组大鼠海马内〔Ca2＋〕i浓度变化,探讨Tet改善VaD大鼠神经功能的分子机制。结果：Nissl染色后,Tet干预组大鼠海马DG区尼氏小体较模型组显著增多;免疫标记发现Tet干预组大鼠DG区神经元内S100B蛋白的表达量明显增多;Tet干预组大鼠海马〔Ca2＋〕i浓度显著下降（p〈0.05）。结论：Tet可改善大鼠脑缺血损伤引起的学习记忆能力障碍,可能与Tet下调S100B蛋白,抑制钙离子有关。
目的：探討粉防己堿（Tet）對血管性癡呆（VaD）大鼠齒狀迴S1000B蛋白錶達的影響以及在血管性癡呆髮病中的作用。方法：運用雙側頸總動脈永久性結扎（2-VO法）建立VaD大鼠模型,Nissl染色觀察海馬齒狀迴（DG）神經元內的尼氏小體的變化,免疫熒光觀察海馬DG區S100B的錶達情況,F1uo-3/AM探針負載後檢測各組大鼠海馬內〔Ca2＋〕i濃度變化,探討Tet改善VaD大鼠神經功能的分子機製。結果：Nissl染色後,Tet榦預組大鼠海馬DG區尼氏小體較模型組顯著增多;免疫標記髮現Tet榦預組大鼠DG區神經元內S100B蛋白的錶達量明顯增多;Tet榦預組大鼠海馬〔Ca2＋〕i濃度顯著下降（p〈0.05）。結論：Tet可改善大鼠腦缺血損傷引起的學習記憶能力障礙,可能與Tet下調S100B蛋白,抑製鈣離子有關。
목적：탐토분방기감（Tet）대혈관성치태（VaD）대서치상회S1000B단백표체적영향이급재혈관성치태발병중적작용。방법：운용쌍측경총동맥영구성결찰（2-VO법）건립VaD대서모형,Nissl염색관찰해마치상회（DG）신경원내적니씨소체적변화,면역형광관찰해마DG구S100B적표체정황,F1uo-3/AM탐침부재후검측각조대서해마내〔Ca2＋〕i농도변화,탐토Tet개선VaD대서신경공능적분자궤제。결과：Nissl염색후,Tet간예조대서해마DG구니씨소체교모형조현저증다;면역표기발현Tet간예조대서DG구신경원내S100B단백적표체량명현증다;Tet간예조대서해마〔Ca2＋〕i농도현저하강（p〈0.05）。결론：Tet가개선대서뇌결혈손상인기적학습기억능력장애,가능여Tet하조S100B단백,억제개리자유관。
Objective：To observe the effect of tetrandrine on the expression of S100B in hippocampal gyrus dentatus（DG）of rats with vascular dementia（VaD）.Methods： SD rats were divided randomly into three groups：model group,sham-operated group and Tet-treated group.The rat model were established by permanent bilateral common carotid artery ligation（2-VO）.After screening Nissl staining and immunofluorescence werer performed to analyze the changes of Nissl bodies and the expressions of S100B level in hippocampus.Also,intracellular calcium was measured by Fluo-3.Results： Nissl bodies were significantly decreased in rats administrated with Tet when compared with those in model group,and the number of immunoreactive positive astrocytes of S100B protein was reduced in the DG of hippocampus in Tet-treated rats.Meanwhile,internal calcium levels in Tet-treated group were markedly lower compared with model group.Conclusion： Tet could ameliorate the learning and memory in VaD by reducing the expression of S100B and internal calcium levels in hippocampus,thereby reduced the extent of brain damage and improved the clinical symptoms of VaD.
