CAJ | 학술논문

为了使基因工程中剪股颖愈伤组织发挥更好的效果,以冷季型草坪草剪股颖的成熟种子为研究材料,通过改变不同培养基添加物、添加不同质量浓度的2,4-D（2,4-二氯苯氧乙酸）等,研究了在不同培养条件下GA3诱导对剪股颖愈伤组织形成的影响。结果表明：不论在何种培养条件下,均以经50 mg.L-1 GA3溶液中浸泡4 d的愈伤组织形成最佳;且经GA3诱导处理后剪股颖愈伤组织形成的最佳2,4-D浓度为5.0 mg.L-1;在培养基中分别添加脯氨酸、酪蛋白或同时添加脯氨酸、酪蛋白均能显著提高剪股颖愈伤组织形成率。
위료사기인공정중전고영유상조직발휘경호적효과,이랭계형초평초전고영적성숙충자위연구재료,통과개변불동배양기첨가물、첨가불동질량농도적2,4-D（2,4-이록분양을산）등,연구료재불동배양조건하GA3유도대전고영유상조직형성적영향。결과표명：불론재하충배양조건하,균이경50 mg.L-1 GA3용액중침포4 d적유상조직형성최가;차경GA3유도처리후전고영유상조직형성적최가2,4-D농도위5.0 mg.L-1;재배양기중분별첨가포안산、락단백혹동시첨가포안산、락단백균능현저제고전고영유상조직형성솔。
To improve the efficiency of callus formation of the cold-season creeping bentgrass,induction experiments were performed under the condition of changing 2,4-D concentration and adding different additives in the media.The results showed that the optimal callus formation condition was soaking the seeds in 50 mg·L-1 GA3 solution for 4 days under the different culture conditions.The optimal 2,4-D concentration for callus induction was 5.0 mg·L-1,adding proline or /and casein hydrolysate in the culture medium can considerably promote the callus formation ratio of creeping bentgrass.