中国动物传染病学报
中國動物傳染病學報
중국동물전염병학보
CHINESE JOURNAL OF VETERINARY PARASITOLOGY
2011年
2期
62-65
,共4页
李凯航%葛菲菲%薛霞%鞠龚讷%周锦萍
李凱航%葛菲菲%薛霞%鞠龔訥%週錦萍
리개항%갈비비%설하%국공눌%주금평
H1N1甲型流感病毒%H1N1亚型猪流感%血凝抑制试验%酶联免疫吸附试验
H1N1甲型流感病毒%H1N1亞型豬流感%血凝抑製試驗%酶聯免疫吸附試驗
H1N1갑형류감병독%H1N1아형저류감%혈응억제시험%매련면역흡부시험
Influenza A virus H1N1 subtype%H1N1 subtype swine influenza%hemagglutination inhibition test%enzyme-linked immunosorbent assay
对2009年H1N1甲型流感流行前后的上海地区养殖场户410份猪血清样品,分别采用血凝抑制试验（hemagglutination inhibition,HI）和酶联免疫吸附试验（enzyme-linked immunosorbent assay,ELISA）进行检测H1N1甲型流感病毒和猪流感病毒（Swine in？uenza virus,SIV）。检测结果表明,除2007年外,2008~2010年猪血清中均存在不同水平的HI抗体,阳性率呈显著上升趋势,且抗体水平与猪群饲养周期及饲养密度正相关,而与猪流感病毒的流行无相关性。
對2009年H1N1甲型流感流行前後的上海地區養殖場戶410份豬血清樣品,分彆採用血凝抑製試驗（hemagglutination inhibition,HI）和酶聯免疫吸附試驗（enzyme-linked immunosorbent assay,ELISA）進行檢測H1N1甲型流感病毒和豬流感病毒（Swine in？uenza virus,SIV）。檢測結果錶明,除2007年外,2008~2010年豬血清中均存在不同水平的HI抗體,暘性率呈顯著上升趨勢,且抗體水平與豬群飼養週期及飼養密度正相關,而與豬流感病毒的流行無相關性。
대2009년H1N1갑형류감류행전후적상해지구양식장호410빈저혈청양품,분별채용혈응억제시험（hemagglutination inhibition,HI）화매련면역흡부시험（enzyme-linked immunosorbent assay,ELISA）진행검측H1N1갑형류감병독화저류감병독（Swine in？uenza virus,SIV）。검측결과표명,제2007년외,2008~2010년저혈청중균존재불동수평적HI항체,양성솔정현저상승추세,차항체수평여저군사양주기급사양밀도정상관,이여저류감병독적류행무상관성。
Detected antibodies of 410 sera derived from different swine herds in shanghai during influenza A virus H1N1 prevalence against influenza A virus H1N1 subtype and SIV by HI test and ELISA,respectively.The results showed that,except in 2007,antibodies against the new influenza A virus H1N1 subtype were at different levels in 2008-2010,and the positive rates showed a significant upward trend;the antibody levels were associated with herd breeding period and density,not were correlated to the SIV prevalence.
