CAJ | 학술논문

在水温9℃～13℃下,将平均体质量0.745±0.3 g的仿刺参（Apostichopus japonicus Selenka）随机投放到50cm×40cm×30cm的塑料水槽内,每箱10头,共分为五组,每组3个平行,采用不同间隔时间投喂每kg添加0.5 mg蛋氨酸硒的饲料：第1、2、3、4组分别间隔0、5、10和15 d投喂一次,第5组投喂不含硒的饲料（对照组）。60d的饲养结果表明：间歇投喂含硒饲料各组仿刺参的成活率和特定生长率差异不显著,但都明显高于对照组;每隔5 d投喂一次含硒饲料的刺参体腔液中SOD活力（78.34U/ml）显著高于其他组（P〈0.05）;谷胱甘肽的浓度（2.87×10-5mol/L）对照组差异不显著,但显著高于其它组（P〈0.05）;连续投喂含硒饲料的仿刺参体内的酸、碱磷酸酶活性（2.42U/ml,5.76U/ml）显著高于其他组（P〈0.05）。建议在生产中宜每日或最多间隔5 d投喂含硒饲料。
재수온9℃～13℃하,장평균체질량0.745±0.3 g적방자삼（Apostichopus japonicus Selenka）수궤투방도50cm×40cm×30cm적소료수조내,매상10두,공분위오조,매조3개평행,채용불동간격시간투위매kg첨가0.5 mg단안산서적사료：제1、2、3、4조분별간격0、5、10화15 d투위일차,제5조투위불함서적사료（대조조）。60d적사양결과표명：간헐투위함서사료각조방자삼적성활솔화특정생장솔차이불현저,단도명현고우대조조;매격5 d투위일차함서사료적자삼체강액중SOD활력（78.34U/ml）현저고우기타조（P〈0.05）;곡광감태적농도（2.87×10-5mol/L）대조조차이불현저,단현저고우기타조（P〈0.05）;련속투위함서사료적방자삼체내적산、감린산매활성（2.42U/ml,5.76U/ml）현저고우기타조（P〈0.05）。건의재생산중의매일혹최다간격5 d투위함서사료。
The effects of feeding regimes on growth and immune indices in juvenile sea cucumber（Apostichopus japonicus） with body weight of 0.745±0.3g fed the diet containing selenomethionine（0.5 mg/kg） were studied at water temperature ranging from 9℃ to 13℃ from March 10,2010,to May 9,2010 in a lab.Total of 150 individuals were selected,randomly stocked into tanks of each 50 cm ×40 cm ×30 cm at a rate of 10 individuals per tank,and fed the diet containing seleniomethionine once daily（Group 1）,once every 5 days（Group 2）,once every 10 days（Group 3）,once every 15 day（Group 4） and once daily for the diet without selenium（Group 5,as control group） with triplication for 60 days.The results showed that the sea cucumber fed the diets containing selenium had significantly higher survival than that in the control group（P 0.05）,even though they had no significant differences in the specific growth compared with the animals in the control group（P 0.05）.There were very significantly higher activities of superoxide dismutase（SOD）（78.34 U/ml） and glutathione（GSH）（2.87×10-5mol/L） in the Group 2 than those in the control group（P 0.05）.No significant difference in the concentration of GSH was found between Group 2 and Group 3,and Group 4（P 0.05）.Compared with the other groups,there were higher acid and alkaline phosphatase activities（2.42 U/ml,and 5.76 U/ml） in Group 1,without significant difference（P 0.05）.It is recommended that the sea cucumber be fed the diet containing seleniumomethionine every day for promotion of survival and immunity.