昆虫学报
昆蟲學報
곤충학보
ACTA ENTOMOLOGICA SINICA
2005年
3期
380-385
,共6页
蔡继峰%刘敏%应斌武%邓仁丽%董建国%张林%陶涛%潘洪富%闫红涛%廖志钢
蔡繼峰%劉敏%應斌武%鄧仁麗%董建國%張林%陶濤%潘洪富%閆紅濤%廖誌鋼
채계봉%류민%응빈무%산인려%동건국%장림%도도%반홍부%염홍도%료지강
食尸性蝇类%物种鉴定%线粒体DNA%细胞色素氧化酶辅酶Ⅰ
食尸性蠅類%物種鑒定%線粒體DNA%細胞色素氧化酶輔酶Ⅰ
식시성승류%물충감정%선립체DNA%세포색소양화매보매Ⅰ
Sarcosaphagous flies%species identification%mitochondrial DNA%cytochrome oxidase Ⅰ
死后不同时间,在尸体上出现不同种类食尸性蝇类的演替规律,可用于准确推断死亡时间.传统上仅依据蝇类形态学特征来判断种属,但由于蝇类的形态结构复杂和种间形态差异微小等特点,对蝇类尤其是对蝇类幼虫的种属鉴别很难.因此应用分子生物学方法对食尸性蝇类及其幼虫进行种属鉴定非常重要.本研究主要是利用此方法对我国西部部分地区常见双翅目食尸性蝇类包括:开普黑蝇、大头金蝇、丝光绿蝇及部分卵,铜绿蝇、棕尾别麻蝇及部分幼虫和蛹的线粒体DNA(mtDNA)上细胞色素氧化酶辅酶Ⅰ(COⅠ)中278 bp的基因序列进行鉴别.除个别蝇类如丝光绿蝇与铜绿蝇外,该方法均能有效地将上述食尸性蝇类鉴定到种属水平.在我国,它将成为法医鉴别食尸性蝇类种属的可靠依据.
死後不同時間,在尸體上齣現不同種類食尸性蠅類的縯替規律,可用于準確推斷死亡時間.傳統上僅依據蠅類形態學特徵來判斷種屬,但由于蠅類的形態結構複雜和種間形態差異微小等特點,對蠅類尤其是對蠅類幼蟲的種屬鑒彆很難.因此應用分子生物學方法對食尸性蠅類及其幼蟲進行種屬鑒定非常重要.本研究主要是利用此方法對我國西部部分地區常見雙翅目食尸性蠅類包括:開普黑蠅、大頭金蠅、絲光綠蠅及部分卵,銅綠蠅、棕尾彆痳蠅及部分幼蟲和蛹的線粒體DNA(mtDNA)上細胞色素氧化酶輔酶Ⅰ(COⅠ)中278 bp的基因序列進行鑒彆.除箇彆蠅類如絲光綠蠅與銅綠蠅外,該方法均能有效地將上述食尸性蠅類鑒定到種屬水平.在我國,它將成為法醫鑒彆食尸性蠅類種屬的可靠依據.
사후불동시간,재시체상출현불동충류식시성승류적연체규률,가용우준학추단사망시간.전통상부의거승류형태학특정래판단충속,단유우승류적형태결구복잡화충간형태차이미소등특점,대승류우기시대승류유충적충속감별흔난.인차응용분자생물학방법대식시성승류급기유충진행충속감정비상중요.본연구주요시이용차방법대아국서부부분지구상견쌍시목식시성승류포괄:개보흑승、대두금승、사광록승급부분란,동록승、종미별마승급부분유충화용적선립체DNA(mtDNA)상세포색소양화매보매Ⅰ(COⅠ)중278 bp적기인서렬진행감별.제개별승류여사광록승여동록승외,해방법균능유효지장상술식시성승류감정도충속수평.재아국,타장성위법의감별식시성승류충속적가고의거.
Sarcosaphagous flies are frequently found on dead bodies shortly after death. Species within this family differ in their developmental times, so an accurate identification of every species is necessary for the correct estimation of the post mortem interval (PMI). Identification of specimens is traditionally performed using their morphological features. The morphological similarity of sarcosaphagous flies especially their eggs, larva and pupae, poses a challenge for forensic entomologists. Therefore a molecular method was established for species identification. In this study, A 278 base pair region of mitochondrial DNA (mtDNA) coding for cytochrome oxidase subunit one (CO Ⅰ ) was investigated for identification of the following forensically important species of sarcosaphagous flies from western China, including Ophyra capensis (Wiedemann), Chrysomya megacephala ( Fabricius), Lucilia sericata ( Meigen), Lucilia cuprina ( Wiedemann), and Boettcherisca peregrina ( Robineau-Desvoidy). The results indicated that the CO Ⅰ region sequenced allowed identification of major species, providing separation of congeneric species with high support. In contrast, the data could not distinguish taxa from the same species group, i. e. the L. sericata and L. cuprina groups because of low sequence divergence.
