CAJ | 학술논문

目的：评价PCR短串联重复序列（PCR-STR）在异基因外周血干细胞移植（Allo-PBSCT）后监测中的应用价值。方法：采集13例造血干细胞移植（HSCT）患者首次术后10、20和30d的口腔颊黏膜上皮细胞及外周静脉血,提取基因组DNA,采用PCR-STR法比较其外周血与口腔黏膜细胞中D7S3048、D4S2639、D14S306、D18S535、F13A01、FESFPS、vWA、CSF1PO、TPOX和THO1等STR位点联合基因型改变。结果：13例HSCT患者的外周血中均发现外源STR基因型。10、20和30d13例患者口腔黏膜细胞与外周血检测的10个STR基因位点均至少有5个不同。结论：对受者外周血与口腔黏膜多个STR基因位点的基因型进行对比,可随时监测HSCT患者植入效果。
목적：평개PCR단천련중복서렬（PCR-STR）재이기인외주혈간세포이식（Allo-PBSCT）후감측중적응용개치。방법：채집13례조혈간세포이식（HSCT）환자수차술후10、20화30d적구강협점막상피세포급외주정맥혈,제취기인조DNA,채용PCR-STR법비교기외주혈여구강점막세포중D7S3048、D4S2639、D14S306、D18S535、F13A01、FESFPS、vWA、CSF1PO、TPOX화THO1등STR위점연합기인형개변。결과：13례HSCT환자적외주혈중균발현외원STR기인형。10、20화30d13례환자구강점막세포여외주혈검측적10개STR기인위점균지소유5개불동。결론：대수자외주혈여구강점막다개STR기인위점적기인형진행대비,가수시감측HSCT환자식입효과。
Aim：To evaluate the value of PCR-STR which applied in monitoring hematopoietic stem cell transplantation（HSCT）.Methods：The peripheral blood and epithelial cell of oral mucosa were collected from 13 patients at 10, 20,and 30 days after the first transplantation. Ten STR loci had been amplified by PCR after the DNA extraction.Results： Exogenous gene was found in the peripheral blood of all the patients.There were at least five STR loci different between the peripheral blood and epithelial cell of mucosa in all of 13 patients.Conclusion：Comparison the DNA genotypes between peripheral blood and oral mucosa could be used to monitor the effect of HSCT.