质谱学报
質譜學報
질보학보
2010年
2期
98-102
,共5页
傅予%梁健%白央%达娃卓玛%白冰如%丁立生
傅予%樑健%白央%達娃卓瑪%白冰如%丁立生
부여%량건%백앙%체왜탁마%백빙여%정립생
尼泊尔黄堇%异喹啉生物碱%串联质谱
尼泊爾黃堇%異喹啉生物堿%串聯質譜
니박이황근%이규람생물감%천련질보
Corydalis hendersonii%isoquinoline alkaloid%tandem mass spectrometry
采用电喷雾串联质谱(ESI-MS/MS)法对尼泊尔黄堇中总生物碱进行分析.由一级质谱中各成分的准分子离子峰获知其相对分子质量,再由二级质谱的裂解碎片推测其结构特征.结合纯品的串联质谱分析,直接鉴定出从尼泊尔黄堇总生物碱中已经分离得到的10种异喹啉生物碱成分:stylopine(1),tetrahydrothalifendine(2),tetrahydroberberrubine(3),scoulerine(4),sinactine(5),protopine(6),cryptopine(7),α-allocryptopine(8),9-methyl-decumbenine C(9)和6-acetonyl-5,6-dihydrosanguinarine(10);另外还初步推测出2种未从该植物中分离得到的异喹啉生物碱成分.
採用電噴霧串聯質譜(ESI-MS/MS)法對尼泊爾黃堇中總生物堿進行分析.由一級質譜中各成分的準分子離子峰穫知其相對分子質量,再由二級質譜的裂解碎片推測其結構特徵.結閤純品的串聯質譜分析,直接鑒定齣從尼泊爾黃堇總生物堿中已經分離得到的10種異喹啉生物堿成分:stylopine(1),tetrahydrothalifendine(2),tetrahydroberberrubine(3),scoulerine(4),sinactine(5),protopine(6),cryptopine(7),α-allocryptopine(8),9-methyl-decumbenine C(9)和6-acetonyl-5,6-dihydrosanguinarine(10);另外還初步推測齣2種未從該植物中分離得到的異喹啉生物堿成分.
채용전분무천련질보(ESI-MS/MS)법대니박이황근중총생물감진행분석.유일급질보중각성분적준분자리자봉획지기상대분자질량,재유이급질보적렬해쇄편추측기결구특정.결합순품적천련질보분석,직접감정출종니박이황근총생물감중이경분리득도적10충이규람생물감성분:stylopine(1),tetrahydrothalifendine(2),tetrahydroberberrubine(3),scoulerine(4),sinactine(5),protopine(6),cryptopine(7),α-allocryptopine(8),9-methyl-decumbenine C(9)화6-acetonyl-5,6-dihydrosanguinarine(10);령외환초보추측출2충미종해식물중분리득도적이규람생물감성분.
Electrospray ionization tandem mass spectrometry(ESI-MS/MS) was used to analyze the total alkaloids from Corydalis hendersonii. Based on the information of pseudo molecular ions and daughter ions, which were obtained from ESI-MS and tandem mass spectrometry, total of 10 main alkaloids are suggested as stylopine(1), tetrahydrothalifendine(2), tetrahydroberberrubine(3), scoulerine(4), sinactine(5), protopine(6), cryptopine(7), α-allocryptopine(8), 9-methyl-decumbenine C(9), and 6-acetonyl-5,6-dihydrosanguinarine(10), respectively.
