CAJ | 학술논문

叶酸偶联的羟脯氨酸-乳酸共聚物(PLLA-PHpr-FA)是一种新型的叶酸受体靶向生物降解聚合物,研究PLLA-PHpr-FA自组装形成胶束的能力及胶束的性质.临界胶束浓度(CMC)用芘荧光探针测定,结果表明,CMC很低并依赖于乳酸/羟脯氨酸的比例.透射电子显微镜(TEM)显示共聚物胶束呈现典型的核/壳结构.动态激光光散射(DLS)测定粒径及粒径分布结果显示,粒径受乳酸/羟脯氨酸比例和丙酮量调控,但粒子几乎不受稀释的影响.用紫外分光光度法测定胶束的载药量和包封率表明,共聚物胶束对疏水性药物的包载较好.因此,PLLA-PHpr-FA胶束可以作为肿瘤靶向的药物载体.
협산우련적간포안산-유산공취물(PLLA-PHpr-FA)시일충신형적협산수체파향생물강해취합물,연구PLLA-PHpr-FA자조장형성효속적능력급효속적성질.림계효속농도(CMC)용비형광탐침측정,결과표명,CMC흔저병의뢰우유산/간포안산적비례.투사전자현미경(TEM)현시공취물효속정현전형적핵/각결구.동태격광광산사(DLS)측정립경급립경분포결과현시,립경수유산/간포안산비례화병동량조공,단입자궤호불수희석적영향.용자외분광광도법측정효속적재약량화포봉솔표명,공취물효속대소수성약물적포재교호.인차,PLLA-PHpr-FA효속가이작위종류파향적약물재체.
The copolymer of L-lactide and 4-hydroxyproline with folate (PLLA-PHpr-FA) is a new kind of folate receptor targeted polylactic acid copolymers. This article aimed to research the self-assembly micelles and the properties of the micelles prepared from PLLA-PHpr-FA. The critical micelle concentration (CMC) was determined with fluorescent probe method, the results showed that the CMCs were quite low and dependent on the molar ratio of lactide/HP. Transmission electron microscope (TEM) indicated that the micelles presented typical core/shell structure. Dynamic light scattering (DLS) was used to measure the particle size and particle size distribution. The size of the particles was found to be regulated by the lactide/HP ratio and the amount of the acetone, but nanoparticle aqueous solution hardly changed over the dilution. The drug contents and loading efficiency of the micelles were investigated with UV spectrophotometry. The result showed that loading efficiency of the micelles for hydrophobic drug was good. Therefore, PLLA-PHpr-FA micelles might serve as a novel potential carrier targeted tumors for drug delivery.