CAJ | 학술논문

目的探讨谷胱甘肽 (GSH) 与顺铂 (CDDP) 联合应用对人肝癌细胞HepG_2细胞增殖及凋亡的影响.方法应用酸性磷酸酶法 (APA) 检测细胞生长抑制率;用倒置显微镜观察经CDDP、GSH处理后的细胞形态学改变;采用流式细胞术研究GSH与CDDP合用对细胞周期的影响;AnnexinV-FITC/PI双染检测细胞凋亡率;罗丹明123染色检测线粒体膜电位 (Δψm) 变化.结果 GSH与CDDP联合应用可以明显抑制HepG_2细胞增殖,癌细胞出现凋亡形态变化,其作用明显高于GSH组与对照组细胞,且不低于CDDP单独给药组;GSH与CDDP联合应用引起Δψm更明显地下降.结论 GSH与CDDP联合用药不降低CDDP对肝癌细胞的作用,其诱导肝癌细胞HepG_2凋亡的作用可能是通过线粒体通路实现的.
목적 탐토곡광감태 (GSH) 여순박 (CDDP) 연합응용대인간암세포HepG_2세포증식급조망적영향.방법 응용산성린산매법 (APA) 검측세포생장억제솔;용도치현미경관찰경CDDP、GSH처리후적세포형태학개변;채용류식세포술연구GSH여CDDP합용대세포주기적영향;AnnexinV-FITC/PI쌍염검측세포조망솔;라단명123염색검측선립체막전위 (Δψm) 변화.결과 GSH여CDDP연합응용가이명현억제HepG_2세포증식,암세포출현조망형태변화,기작용명현고우GSH조여대조조세포,차불저우CDDP단독급약조;GSH여CDDP연합응용인기Δψm경명현지하강.결론 GSH여CDDP연합용약불강저CDDP대간암세포적작용,기유도간암세포HepG_2조망적작용가능시통과선립체통로실현적.
Objective To explore the effect of glutathione (GSH) combined with cisplatin (CDDP) on the proliferation and apoptosis of HepG2 cells. Methods Acid phosphatase assay (APA) was used to determine the inhibition rate of cells. Inverted microscope was used to observe the morphological changes of cells after CDDP and GSH treatment. Flow cytometry was used to study the effect of GSH combined with CDDP on the cell cycle. AnnexinV-FITC/PI was carried out to evaluate the apoptosis. The change of mitochondrial membrane potential (Δψm) was observed by means of Rhodamine123. Results GSH combined with CDDP could obviously inhibit the proliferation and apoptosis of HepG_2 and the apoptosis rate in GSH combined with CDDP group were significantly higher than those of GSH group and the control group, moreover, were not lower than those of CDDP alone group. GSH combined with CDDP could lead to more obvious decline of Δψm than CDDP. Conclusions GSH combined with CDDP could not reduce the inhibition effect of CDDP on HepG_2. The mitochondia pathway plays an important role in inducing apoptosis of HepG_2.