中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2010年
4期
487-490
,共4页
徐艳岩%安郁宽%李波清%李京敏
徐豔巖%安鬱寬%李波清%李京敏
서염암%안욱관%리파청%리경민
谷胱甘肽%顺铂%肝癌细胞%凋亡%细胞周期
穀胱甘肽%順鉑%肝癌細胞%凋亡%細胞週期
곡광감태%순박%간암세포%조망%세포주기
Glutathione%Cisplatin%Hepatoma%Apoptosis%Cell cycle
目的 探讨谷胱甘肽 (GSH) 与顺铂 (CDDP) 联合应用对人肝癌细胞HepG_2细胞增殖及凋亡的影响.方法 应用酸性磷酸酶法 (APA) 检测细胞生长抑制率;用倒置显微镜观察经CDDP、GSH处理后的细胞形态学改变;采用流式细胞术研究GSH与CDDP合用对细胞周期的影响;AnnexinV-FITC/PI双染检测细胞凋亡率;罗丹明123染色检测线粒体膜电位 (Δψm) 变化.结果 GSH与CDDP联合应用可以明显抑制HepG_2细胞增殖,癌细胞出现凋亡形态变化,其作用明显高于GSH组与对照组细胞,且不低于CDDP单独给药组;GSH与CDDP联合应用引起Δψm更明显地下降.结论 GSH与CDDP联合用药不降低CDDP对肝癌细胞的作用,其诱导肝癌细胞HepG_2凋亡的作用可能是通过线粒体通路实现的.
目的 探討穀胱甘肽 (GSH) 與順鉑 (CDDP) 聯閤應用對人肝癌細胞HepG_2細胞增殖及凋亡的影響.方法 應用痠性燐痠酶法 (APA) 檢測細胞生長抑製率;用倒置顯微鏡觀察經CDDP、GSH處理後的細胞形態學改變;採用流式細胞術研究GSH與CDDP閤用對細胞週期的影響;AnnexinV-FITC/PI雙染檢測細胞凋亡率;囉丹明123染色檢測線粒體膜電位 (Δψm) 變化.結果 GSH與CDDP聯閤應用可以明顯抑製HepG_2細胞增殖,癌細胞齣現凋亡形態變化,其作用明顯高于GSH組與對照組細胞,且不低于CDDP單獨給藥組;GSH與CDDP聯閤應用引起Δψm更明顯地下降.結論 GSH與CDDP聯閤用藥不降低CDDP對肝癌細胞的作用,其誘導肝癌細胞HepG_2凋亡的作用可能是通過線粒體通路實現的.
목적 탐토곡광감태 (GSH) 여순박 (CDDP) 연합응용대인간암세포HepG_2세포증식급조망적영향.방법 응용산성린산매법 (APA) 검측세포생장억제솔;용도치현미경관찰경CDDP、GSH처리후적세포형태학개변;채용류식세포술연구GSH여CDDP합용대세포주기적영향;AnnexinV-FITC/PI쌍염검측세포조망솔;라단명123염색검측선립체막전위 (Δψm) 변화.결과 GSH여CDDP연합응용가이명현억제HepG_2세포증식,암세포출현조망형태변화,기작용명현고우GSH조여대조조세포,차불저우CDDP단독급약조;GSH여CDDP연합응용인기Δψm경명현지하강.결론 GSH여CDDP연합용약불강저CDDP대간암세포적작용,기유도간암세포HepG_2조망적작용가능시통과선립체통로실현적.
Objective To explore the effect of glutathione (GSH) combined with cisplatin (CDDP) on the proliferation and apoptosis of HepG2 cells. Methods Acid phosphatase assay (APA) was used to determine the inhibition rate of cells. Inverted microscope was used to observe the morphological changes of cells after CDDP and GSH treatment. Flow cytometry was used to study the effect of GSH combined with CDDP on the cell cycle. AnnexinV-FITC/PI was carried out to evaluate the apoptosis. The change of mitochondrial membrane potential (Δψm) was observed by means of Rhodamine123. Results GSH combined with CDDP could obviously inhibit the proliferation and apoptosis of HepG_2 and the apoptosis rate in GSH combined with CDDP group were significantly higher than those of GSH group and the control group, moreover, were not lower than those of CDDP alone group. GSH combined with CDDP could lead to more obvious decline of Δψm than CDDP. Conclusions GSH combined with CDDP could not reduce the inhibition effect of CDDP on HepG_2. The mitochondia pathway plays an important role in inducing apoptosis of HepG_2.