遗传
遺傳
유전
HEREDITAS(BEIJING)
2009年
12期
1248-1258
,共11页
曹广力%薛仁宇%朱越雄%魏育红%贡成良
曹廣力%薛仁宇%硃越雄%魏育紅%貢成良
조엄력%설인우%주월웅%위육홍%공성량
家蚕%氨酰-tRNA合成酶%核基因%线粒体%电子克隆
傢蠶%氨酰-tRNA閤成酶%覈基因%線粒體%電子剋隆
가잠%안선-tRNA합성매%핵기인%선립체%전자극륭
Bombyxmori%aminoacyl-tRNA synthetase(aaRS)%nuclear gene%mitochondrial%in silico cloning
为了探讨家蚕氨酰-tRNA合成酶(BmaaRS)基因的数目、种类、结构及起源,利用家蚕基因组数据和EST数据进行了BmaaRS基因的电子克隆,结果表明,家蚕核基因组中含有2套不同的aaRS核基因,分别编码线粒体和细胞质BmaaRS,但编码线粒体BmSerRS的基因有2个,可能缺少编码细胞质的BmHisRS基因和编码线粒体的BmGlnRS、BmLysRS、BmGlyRS、BmGlyRS和BmThrRS基因,这些基因的功能可能由具有相似功能的其他蛋白完成,或通过某个BmaaRS基因的可变剪接分别形成不同功能的BmaaRS.EST证据表明,BmaaRS基因存在不同形式的可变剪接;BmaaRS氨基酸序列的相似性及二、三级结构分析表明部分BmaaRS存在结构域的扩增,有些不同的BmaaRS具有相同结构域,相同功能的BmaaRS具有相似的三级结构;进化分析表明,BmaaRS为2套不同来源的BmaaRS基因编码,细胞质和线粒体BmaaRS的起源不同.
為瞭探討傢蠶氨酰-tRNA閤成酶(BmaaRS)基因的數目、種類、結構及起源,利用傢蠶基因組數據和EST數據進行瞭BmaaRS基因的電子剋隆,結果錶明,傢蠶覈基因組中含有2套不同的aaRS覈基因,分彆編碼線粒體和細胞質BmaaRS,但編碼線粒體BmSerRS的基因有2箇,可能缺少編碼細胞質的BmHisRS基因和編碼線粒體的BmGlnRS、BmLysRS、BmGlyRS、BmGlyRS和BmThrRS基因,這些基因的功能可能由具有相似功能的其他蛋白完成,或通過某箇BmaaRS基因的可變剪接分彆形成不同功能的BmaaRS.EST證據錶明,BmaaRS基因存在不同形式的可變剪接;BmaaRS氨基痠序列的相似性及二、三級結構分析錶明部分BmaaRS存在結構域的擴增,有些不同的BmaaRS具有相同結構域,相同功能的BmaaRS具有相似的三級結構;進化分析錶明,BmaaRS為2套不同來源的BmaaRS基因編碼,細胞質和線粒體BmaaRS的起源不同.
위료탐토가잠안선-tRNA합성매(BmaaRS)기인적수목、충류、결구급기원,이용가잠기인조수거화EST수거진행료BmaaRS기인적전자극륭,결과표명,가잠핵기인조중함유2투불동적aaRS핵기인,분별편마선립체화세포질BmaaRS,단편마선립체BmSerRS적기인유2개,가능결소편마세포질적BmHisRS기인화편마선립체적BmGlnRS、BmLysRS、BmGlyRS、BmGlyRS화BmThrRS기인,저사기인적공능가능유구유상사공능적기타단백완성,혹통과모개BmaaRS기인적가변전접분별형성불동공능적BmaaRS.EST증거표명,BmaaRS기인존재불동형식적가변전접;BmaaRS안기산서렬적상사성급이、삼급결구분석표명부분BmaaRS존재결구역적확증,유사불동적BmaaRS구유상동결구역,상동공능적BmaaRS구유상사적삼급결구;진화분석표명,BmaaRS위2투불동래원적BmaaRS기인편마,세포질화선립체BmaaRS적기원불동.
For further research on number,type.composition and origin of Bombyx mori aminoacyl-tRNA synthetase (BmaaRS)genes,in silico cloning was performed with Bombyx mori genomic and EST databases.There might be two different sets of aaRS nuclear gene in Bombxy nori genome,which encode mitochondrial BmaaRS and cytoplasmic BmaaRS,respectively.Among BmaaRS genes.there were 2 genes encoding mitochondrial BmSerRS,but no genes encoding cytoplasmic BmHisRS and mitochondrial BmGlnRS,BmLysRS,BmGlyRS,and BmThrRS.The functions of these absent genes could be directly replaced by other proteins with similar functions,or might undergo their distinct BmaaRS functions based on the alternative splice of one certain BmaaRS mRNA.Evidence of EST indicated that BmaaRS performed different alternative splicing patterns.The homology comparison and advanced structural analysis of BmaaRS demonstrated the existence of extended domains of BmaaRS.This is because some different BmaaRSs contained similar domain.Moreover,BmaaRSs with similar functions possessed the similax tertiary structure.Phylogenetic analysis revealed that BmaaRS encoded by two various sources of BmaaRS genes.Mitochondrial and cytoplasmic BmaaRS had different origin.
