上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
12期
1482-1486
,共5页
伍宏彪%张鹏%李小强%吴巨钢%俞继卫%姜波健
伍宏彪%張鵬%李小彊%吳巨鋼%俞繼衛%薑波健
오굉표%장붕%리소강%오거강%유계위%강파건
胃癌%c-met%e2f-1%Ki-67%预后
胃癌%c-met%e2f-1%Ki-67%預後
위암%c-met%e2f-1%Ki-67%예후
gastric cancer%c-met%e2f-1%Ki-67%prognosis
目的 检测肝细胞生长因子受体c-met、转录因子e2f-1和Ki-67抗原在胃癌组织中的表达,探讨三者的相关性及与临床病理特征和预后的关系.方法 免疫组织化学染色法检测86例胃癌患者手术标本中c-met、e2f-1和Ki-67的表达,单因素与多因素法分析其与临床病理特征的关系及三者的相关性,Log rank检验分析其与患者预后的关系.结果 c-met、e2f-1和Ki-67在胃癌组织中均广泛表达.c-met的高表达与Ki-67高表达、生存期较短、肿瘤位置较高、淋巴结转移率较高、累及淋巴站较远及TNM分期较晚有关(P<0.05);e2f-1的低表达与肿瘤直径较大、TNM分期较晚、浸润较深、淋巴结转移率较高、累及淋巴站较远有关(P<0.05).多因素分析显示,浸润深度、累及淋巴站、TNM分期和Ki-67表达是c-met表达的独立相关因素,而年龄和生存期是e2f-1表达的独立相关因素.Log rank检验显示,与术后生存相关的因素有c-met、e2f-1和Ki-67表达、累及淋巴站、肿瘤直径、肿瘤位置、淋巴结转移率、浸润深度和TNM分期.结论 c-met阳性表达的胃癌患者生存期显著缩短.e2f-1在早期胃癌中高表达,晚期胃癌的进展可能与e2f-1表达降低有关.
目的 檢測肝細胞生長因子受體c-met、轉錄因子e2f-1和Ki-67抗原在胃癌組織中的錶達,探討三者的相關性及與臨床病理特徵和預後的關繫.方法 免疫組織化學染色法檢測86例胃癌患者手術標本中c-met、e2f-1和Ki-67的錶達,單因素與多因素法分析其與臨床病理特徵的關繫及三者的相關性,Log rank檢驗分析其與患者預後的關繫.結果 c-met、e2f-1和Ki-67在胃癌組織中均廣汎錶達.c-met的高錶達與Ki-67高錶達、生存期較短、腫瘤位置較高、淋巴結轉移率較高、纍及淋巴站較遠及TNM分期較晚有關(P<0.05);e2f-1的低錶達與腫瘤直徑較大、TNM分期較晚、浸潤較深、淋巴結轉移率較高、纍及淋巴站較遠有關(P<0.05).多因素分析顯示,浸潤深度、纍及淋巴站、TNM分期和Ki-67錶達是c-met錶達的獨立相關因素,而年齡和生存期是e2f-1錶達的獨立相關因素.Log rank檢驗顯示,與術後生存相關的因素有c-met、e2f-1和Ki-67錶達、纍及淋巴站、腫瘤直徑、腫瘤位置、淋巴結轉移率、浸潤深度和TNM分期.結論 c-met暘性錶達的胃癌患者生存期顯著縮短.e2f-1在早期胃癌中高錶達,晚期胃癌的進展可能與e2f-1錶達降低有關.
목적 검측간세포생장인자수체c-met、전록인자e2f-1화Ki-67항원재위암조직중적표체,탐토삼자적상관성급여림상병리특정화예후적관계.방법 면역조직화학염색법검측86례위암환자수술표본중c-met、e2f-1화Ki-67적표체,단인소여다인소법분석기여림상병리특정적관계급삼자적상관성,Log rank검험분석기여환자예후적관계.결과 c-met、e2f-1화Ki-67재위암조직중균엄범표체.c-met적고표체여Ki-67고표체、생존기교단、종류위치교고、림파결전이솔교고、루급림파참교원급TNM분기교만유관(P<0.05);e2f-1적저표체여종류직경교대、TNM분기교만、침윤교심、림파결전이솔교고、루급림파참교원유관(P<0.05).다인소분석현시,침윤심도、루급림파참、TNM분기화Ki-67표체시c-met표체적독립상관인소,이년령화생존기시e2f-1표체적독립상관인소.Log rank검험현시,여술후생존상관적인소유c-met、e2f-1화Ki-67표체、루급림파참、종류직경、종류위치、림파결전이솔、침윤심도화TNM분기.결론 c-met양성표체적위암환자생존기현저축단.e2f-1재조기위암중고표체,만기위암적진전가능여e2f-1표체강저유관.
Objective To detect the expression of c-met (hepatocyte growth factor receptor), e2f-1 (transcription factor) and Ki-67 in tissues of gastric cancer, explore the relationship among them, and investigate their correlationship with clinicopathological characteristics and prognosis. Methods The tissue samples of gastric cancer from 86 patients with radical resection were subjected to immunohistochemical staining, and the expression of c-met, e2f-1 and Ki-67 was detected. The relationship between the clinicopathological characteristics and the expression of c-met, e2f-1 and Ki-67, and that among the expression of c-met, e2f-1 and Ki-67 were explored by univariate and multivariate data analysis. And the relationship between prognosis and expression of c-met, e2f-1 and Ki-67 was analysed by Log rank test. Results c-met, e2f-1 and Ki-67 were widely expressed in tissues of gastric cancer. The higher expression of c-met was associated with higher expression of Ki-67, shorter survival time, upper tumor location, higher lymph node metastasis rate, higher N stage and TNM stage (P<0.05), and the lower expression of e2f-1 was associated with larger tumor diameter, higher TNM stage, deeper invasion, higher lymph node metastasis rate and higher N stage (P<0.05). The multivariate analysis revealed that depth of invasion, N stage, TNM stage and expression of Ki-67 were independent factors for positive expression of c-met, and age and survival time were independent factors for positive expression of e2f-1. Log rank test demonstrated that the factors related to survival included expression of c-met, e2f-1 and Ki-67, N stage, tumor diameter, tumor location, lymph node metastasis rate, depth of invasion and TNM stage. Conclusion c-met is an indicator for malignant behavior and poorer prognosis of gastric cancer. There is a higher expression of e2f-1 in early gastric cancer, while advanced gastric cancer may be associated with a lower expression of e2f-1.
