CAJ | 학술논문

合成了一种新的荧光探针试剂香草醛缩苯胺,利用元素分析、红外光谱等手段对探针试剂进行结构表征;结合邻苯三酚的自氧化作用,建立了一种荧光法测定超氧阴离子自由基(O_2~(-·))的新方法.该方法具有操作简单、灵敏度高和选择性好等特点.邻苯三酚线性范围为4.0×10~(-6)～1.0×10~(-5) mol·~(-1).检出限为2.0×10~(-7) mol·~(-1).方法用于大蒜等样品中超氧化物歧化酶(SOD)活性检测,结果满意.
합성료일충신적형광탐침시제향초철축분알,이용원소분석、홍외광보등수단대탐침시제진행결구표정;결합린분삼분적자양화작용,건립료일충형광법측정초양음리자자유기(O_2~(-·))적신방법.해방법구유조작간단、령민도고화선택성호등특점.린분삼분선성범위위4.0×10~(-6)～1.0×10~(-5) mol·~(-1).검출한위2.0×10~(-7) mol·~(-1).방법용우대산등양품중초양화물기화매(SOD)활성검측,결과만의.
A new fluorescent reagent N-[3-methoxy-4-hydroxyphenylmenthylidene]-aniline was synthesized, and its structural characters was characterized by elemental analysis and infrared spectroscopy. With the new synthesized fluorescence reagent, a novel fluorescence quenching method for determination of superoxide radical was developed by coupling the reaction of pyrogallic auto-oxidation reaction. The method is simple and sensitive. The linear range and detection limit are 4.0×10~(-6)～1.0×10~(-5) mol·~(-1) and 2.0×10~(-7) mol·~(-1), respectively. The approach has been applied to determine superoxide radical in garlic sample with satisfying results.