CAJ | 학술논문

隐花色素(cryptochrome,简称CRY)是与DNA光解酶氨基酸序列高度同源的黄素蛋白,但不具有光解酶活性.拟南芥的隐花色素家族中隐花色素1(CRY1)主要抑制下胚轴伸长,隐花色素2(CRY2)主要调节光周期开花,并且这两个光受体具有部分重叠的功能.cry1cry2双突变体在长日条件下比野生型晚开花.采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D (suppressor of cry1cry2#17-Dominant).TAIL-PCR结果表明,scc17-D突变体的T-DNA插入在第一条染色体上的At1g25440和At1g25450两个基因之间.scc17-D突变体表现出植株矮化、叶片变小、果荚变小和子叶表皮细胞形状发生改变等性状.
은화색소(cryptochrome,간칭CRY)시여DNA광해매안기산서렬고도동원적황소단백,단불구유광해매활성.의남개적은화색소가족중은화색소1(CRY1)주요억제하배축신장,은화색소2(CRY2)주요조절광주기개화,병차저량개광수체구유부분중첩적공능.cry1cry2쌍돌변체재장일조건하비야생형만개화.채용격활표첨적방법,재cry1cry2쌍돌변체배경하사선도일개조개화돌변체scc17-D (suppressor of cry1cry2#17-Dominant).TAIL-PCR결과표명,scc17-D돌변체적T-DNA삽입재제일조염색체상적At1g25440화At1g25450량개기인지간.scc17-D돌변체표현출식주왜화、협편변소、과협변소화자협표피세포형상발생개변등성상.
Cryptochromes are flavoproteins that share similarity with the sequence of amino acids in DNA photolyase but lack photolyase activity. Among members of cryptochromes gene family in Arabidopsis thaliana, cryptochrome1 (CRY1) and CRY2 primarily mediate blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation, respectively, although their functions do overlap. The cry1cry2 double mutant flowered later than wild-type under long day conditions. Through activation tagging, we identified an early flowering mutant called scc17-D (suppressor of cry1cry2#17-Dominant). TAIL-PCR results showed that a T-DNA insertion located between At1g25440 and At1g25450 in the first chromosome is responsible for the scc17-D mutant. The scc17-D mutant displayed dwarfism, reduction in leaf size, reduced length of siliques, and the altered shape of cotyledon pavement cells.