CAJ | 학술논문

目的:探索简捷细胞培养方法并观察该方法对癌细胞特性的影响.方法:将复苏的OC-3-VGH卵巢癌细胞株不经洗涤直接移至细胞培养瓶,加入10ml RPMI-1640培养液,放入培养箱,余同传统方法,观察细胞生长情况;取细胞悬液,以细胞数4×106/ml,0.2ml/只接种至BALB/c雌性裸小鼠皮下,2个月后处死,取肿瘤组织制片,并与传统培养方法结果进行比较.结果:两种方法培养的细胞均生长活跃,皮下接种7d左右,裸鼠长出肿瘤,组织学检查未见明显差异.结论:改良细胞培养方法减少了传统方法的繁琐步骤,方便细胞培养.
목적:탐색간첩세포배양방법병관찰해방법대암세포특성적영향.방법:장복소적OC-3-VGH란소암세포주불경세조직접이지세포배양병,가입10ml RPMI-1640배양액,방입배양상,여동전통방법,관찰세포생장정황;취세포현액,이세포수4×106/ml,0.2ml/지접충지BALB/c자성라소서피하,2개월후처사,취종류조직제편,병여전통배양방법결과진행비교.결과:량충방법배양적세포균생장활약,피하접충7d좌우,라서장출종류,조직학검사미견명현차이.결론:개량세포배양방법감소료전통방법적번쇄보취,방편세포배양.
Objective:To explore a modified cell culture method and observe its influences on characteristics of cell line OC-3-VGH of ovarian cancer. Methods:Resuscitated cells of ovarian cancer cell line OC-3-VGH were moved to cell culture bottle directly without washing, and put into incubaton after adding lOml RPMI - 1640 nutrient solution. The other processes were consistent with the traditional method, and the situation of cell growth was observed. Cell suspension (4 × 10<'6>/ml) was taken out and inoculated to BALB/c female nude mice (0.2ml per mouse). All mice were executed after 2 months, and tumor tis-sues were taken out. Tissue sections were prepared and compared with the traditional culture method. Results:Active cell growths were presented in the 2 cell culture methods. Tumor tissues were observed almost at the 7th day after hypodermic inoc-ulation. And there were no significant differences in histological examination. Conclusion:The modified cell culture method of this study is more convenient for cell culture than traditional method with its simple procedures.