中国医院药学杂志
中國醫院藥學雜誌
중국의원약학잡지
CHINESE JOURNAL OF HOSPITAL PHARMACY
2008年
22期
1909-1912
,共4页
韦世秀%刘成军%李牡艳%宋慧%李佳荃
韋世秀%劉成軍%李牡豔%宋慧%李佳荃
위세수%류성군%리모염%송혜%리가전
广西眼镜蛇毒%神经生长因子%鼻咽癌%CNE-2细胞%细胞凋亡
廣西眼鏡蛇毒%神經生長因子%鼻嚥癌%CNE-2細胞%細胞凋亡
엄서안경사독%신경생장인자%비인암%CNE-2세포%세포조망
目的:研究广西眼镜蛇毒神经生长因子(Nerve growth factor,NGF)对人鼻咽癌CNE-2细胞增殖的抑制作用和诱导细胞凋亡.方法:应用三磷酸腺苷-生物荧光肿瘤化疗药物敏感试验法(ATP-TCA法)测定不同质量浓度(0.0125,0.025,0.05,0.1,0.2 g·L)NGF处理24,48,72,96 h对人鼻咽癌CNE-2生长的抑制率;Hoechst 33342荧光染色观察凋亡细胞的形态;琼脂糖凝胶电泳测定DNA断裂状况;流式细胞术检测细胞周期变化和细胞凋亡率.结果:不同浓度的NGF能抑制CNE-2细胞增殖,并呈浓度-时间效应关系,作用24,48,72,96 h的半数抑制浓度(IC50)分别为0.213,0.095,0.048,0.033 g·L-1;经NGF(0.1 g·L-1)处理细胞48 h后,荧光染色呈现典型的细胞凋亡形态学特征;DNA凝胶电泳可见凋亡细胞特有的DNA片段;0.05.0.1,0.2 g·L-1NGF作用48 h后,CNE-2细胞被阻滞于G0/G1期,细胞凋亡率分别为(28.2±2.0)%、(39.9±1.9)%、(50.3±1.3)%.结论:NGF通过诱导人鼻咽癌CNE-2细胞凋亡而产生抗鼻咽癌活性.
目的:研究廣西眼鏡蛇毒神經生長因子(Nerve growth factor,NGF)對人鼻嚥癌CNE-2細胞增殖的抑製作用和誘導細胞凋亡.方法:應用三燐痠腺苷-生物熒光腫瘤化療藥物敏感試驗法(ATP-TCA法)測定不同質量濃度(0.0125,0.025,0.05,0.1,0.2 g·L)NGF處理24,48,72,96 h對人鼻嚥癌CNE-2生長的抑製率;Hoechst 33342熒光染色觀察凋亡細胞的形態;瓊脂糖凝膠電泳測定DNA斷裂狀況;流式細胞術檢測細胞週期變化和細胞凋亡率.結果:不同濃度的NGF能抑製CNE-2細胞增殖,併呈濃度-時間效應關繫,作用24,48,72,96 h的半數抑製濃度(IC50)分彆為0.213,0.095,0.048,0.033 g·L-1;經NGF(0.1 g·L-1)處理細胞48 h後,熒光染色呈現典型的細胞凋亡形態學特徵;DNA凝膠電泳可見凋亡細胞特有的DNA片段;0.05.0.1,0.2 g·L-1NGF作用48 h後,CNE-2細胞被阻滯于G0/G1期,細胞凋亡率分彆為(28.2±2.0)%、(39.9±1.9)%、(50.3±1.3)%.結論:NGF通過誘導人鼻嚥癌CNE-2細胞凋亡而產生抗鼻嚥癌活性.
목적:연구엄서안경사독신경생장인자(Nerve growth factor,NGF)대인비인암CNE-2세포증식적억제작용화유도세포조망.방법:응용삼린산선감-생물형광종류화료약물민감시험법(ATP-TCA법)측정불동질량농도(0.0125,0.025,0.05,0.1,0.2 g·L)NGF처리24,48,72,96 h대인비인암CNE-2생장적억제솔;Hoechst 33342형광염색관찰조망세포적형태;경지당응효전영측정DNA단렬상황;류식세포술검측세포주기변화화세포조망솔.결과:불동농도적NGF능억제CNE-2세포증식,병정농도-시간효응관계,작용24,48,72,96 h적반수억제농도(IC50)분별위0.213,0.095,0.048,0.033 g·L-1;경NGF(0.1 g·L-1)처리세포48 h후,형광염색정현전형적세포조망형태학특정;DNA응효전영가견조망세포특유적DNA편단;0.05.0.1,0.2 g·L-1NGF작용48 h후,CNE-2세포피조체우G0/G1기,세포조망솔분별위(28.2±2.0)%、(39.9±1.9)%、(50.3±1.3)%.결론:NGF통과유도인비인암CNE-2세포조망이산생항비인암활성.
