CAJ | 학술논문

目的:探讨Beh(c)et病患者结节红斑中CD40/CD40L分子的表达及意义.方法:采用免疫组化方法对5例Beh(c)et病活动期患者腿或臂部结节红斑中CD4,CD8,CD19,CD68,HLA-DR,CD40和CD40L分子表达的情况进行了检测,并用双重染色方法探讨了细胞来源.结果:Behcet病结节红斑中表皮约90%的细胞表达CD40,但无CD40L表达.真皮和皮下表达CD4,CD8,CD19,CD68,HLA-DR,CD1a,CD40L和CD40的细胞均显著增多.双染结果显示,45%的CD4+T细胞表达CD40L分子.100%CD68+细胞表达CD40分子.59.2%的HLA-DR+细胞表达CD40分子.结论:CD40L分子主要表达于CD4+T细胞,CD40分子主要表达于单核巨噬细胞、抗原提呈细胞和角朊细胞.CD40/CD40L通路可诱导巨噬细胞和T淋巴细胞的功能和活性,对Beh(c)et病患者结节红斑的发生、炎症的持续和扩大起重要作用.
목적:탐토Beh(c)et병환자결절홍반중CD40/CD40L분자적표체급의의.방법:채용면역조화방법대5례Beh(c)et병활동기환자퇴혹비부결절홍반중CD4,CD8,CD19,CD68,HLA-DR,CD40화CD40L분자표체적정황진행료검측,병용쌍중염색방법탐토료세포래원.결과:Behcet병결절홍반중표피약90%적세포표체CD40,단무CD40L표체.진피화피하표체CD4,CD8,CD19,CD68,HLA-DR,CD1a,CD40L화CD40적세포균현저증다.쌍염결과현시,45%적CD4+T세포표체CD40L분자.100%CD68+세포표체CD40분자.59.2%적HLA-DR+세포표체CD40분자.결론:CD40L분자주요표체우CD4+T세포,CD40분자주요표체우단핵거서세포、항원제정세포화각원세포.CD40/CD40L통로가유도거서세포화T림파세포적공능화활성,대Beh(c)et병환자결절홍반적발생、염증적지속화확대기중요작용.
· AIM: To investigate the expression and the possible implication of CD40/CD40L costimulatory molecules in erythema nodosum of patients with Beh(c)et's disease.· METHODS: Sampling was done from erythema nodosum of 5 patients with Beh(c)et's disease and normal skin of 2 healthy individuals. Immunohistochemical staining was performed to examine the expression of CD4, CD8, CD19, CD68, HLA-DR,CD40 and CD40L molecules in the obtained tissues.· RESULTS: Approximately 90% of epidermic cells in erythema nodosum expressed CD40 molecule. In the dermis and subcutaneous tissue, a significantly increased number of CD4+Tcells, CD8+Tcells, CD19+cells, CD68+cells, HLA-DR+cells,CD40L+cells, and CD40+cells were observed in the erythema nodosum as compared with that in normal skin. Double staining showed that CD40L molecules were expressed on 45% of CD4+T cells. CD40 molecules were expressed on 100% CD68+ cells and 59.2% of HLA-DR+cells respectively.· CONCLUSION: A number of CD40/CD40L costimulatory molecules are upreguiated in the erythema nodosum of patients with Behcet's disease.