CAJ | 학술논문

用膜片钳技术首次研究了三氟氯氰菊酯对离体培养的棉铃虫中枢神经细胞延迟整流钾通道电流的影响.结果表明,药物作用前有81%和39%的细胞的通道分别在-30 mV和-40 mV激活(n=21).三氟氯氰菊酯(10-5mmol/L)作用15 min后,有63%和38%细胞的通道分别在-40 mV和-50 mV激活(n=8);作用1 min后电流幅值明显降低,抑制率达到了37.7%(n=19);加药后激活曲线明显左移且Vh值变化显著,但k值没有明显变化.实验结果说明,三氟氯氰菊酯作用后,通道更容易激活,但显著抑制电流峰值,导致神经敏感性降低,棉铃虫中枢神经细胞钾通道也是拟除虫菊酯类药物的作用靶标之一.
용막편겸기술수차연구료삼불록청국지대리체배양적면령충중추신경세포연지정류갑통도전류적영향.결과표명,약물작용전유81%화39%적세포적통도분별재-30 mV화-40 mV격활(n=21).삼불록청국지(10-5mmol/L)작용15 min후,유63%화38%세포적통도분별재-40 mV화-50 mV격활(n=8);작용1 min후전류폭치명현강저,억제솔체도료37.7%(n=19);가약후격활곡선명현좌이차Vh치변화현저,단k치몰유명현변화.실험결과설명,삼불록청국지작용후,통도경용역격활,단현저억제전류봉치,도치신경민감성강저,면령충중추신경세포갑통도야시의제충국지류약물적작용파표지일.
The effects of cyhalothrin on the delayed rectifier potassium current in the central neurons of Helicoverpa armigera were studied using the patch clamp technique for the first time. The results showed that before application of cyhalothrin, 81% and 39% cells were activated at -30 mV and -40 mV (n = 21), respectively. In 15 min after cyhalothrin (10-5 mmol/L) application, 63% and 38% cells were activated at - 40 mV and - 50 mV ( n = 8),respectively. The amplitude of the current decreased significantly after application of cyhalothrin and the inhibition percentage reached 37.7% in 1 min (n = 19). After the application, activation curve shifted to the negative direction and the value of Vh changed significantly, but the k value did not change remarkably. In conclusion, the results suggest that under the action of cyhalothrin, the potassium channels can be activated more easily and current amplitude can be inhibited significantly, this is related to the nervous insensitivity, and potassium channels of Helicoverpa armigera central neurons are the action targets of pyrethroid insecticides.