CAJ | 학술논문

目的评价灯盏细辛的抗癌变效果并动态观察其对癌血管生成的影响。方法用Salley法诱导金黄地鼠颊囊白斑癌变，以灯盏细辛干预，采用墨汁灌注图像分析、微血管树脂铸形、α-SMA免疫组化染色及组织病理对照等多种方法联合动态观察其变化规律。结果灯盏细辛组较模型组白斑癌变率下降1倍，正常细胞率是模型组的近4倍。α-SMA值明显高于模型组(P<0.001)。血管面积与血管密度比值两组间相比P>0.05，但树脂微血管铸形显示形态和空间构筑近似于空白对照组。结论灯盏细辛有确切的防止白斑癌变的功效。该药对白斑癌变过程中的血管增生和扩张无明显影响，但对血管构形、空间配置和血管壁的完整性有保护作用，可能是活血化瘀类中药发挥抗癌功效的“良性血管生成”基础。
목적평개등잔세신적항암변효과병동태관찰기대암혈관생성적영향。방법용Salley법유도금황지서협낭백반암변，이등잔세신간예，채용묵즙관주도상분석、미혈관수지주형、α-SMA면역조화염색급조직병리대조등다충방법연합동태관찰기변화규률。결과등잔세신조교모형조백반암변솔하강1배，정상세포솔시모형조적근4배。α-SMA치명현고우모형조(P<0.001)。혈관면적여혈관밀도비치량조간상비P>0.05，단수지미혈관주형현시형태화공간구축근사우공백대조조。결론등잔세신유학절적방지백반암변적공효。해약대백반암변과정중적혈관증생화확장무명현영향，단대혈관구형、공간배치화혈관벽적완정성유보호작용，가능시활혈화어류중약발휘항암공효적“량성혈관생성”기출。
Objective　To evaluate the effect of resisting canceration of herba erigerontis and that of angiopoiesis. Methods　Carcinomatous conversions of golden hamster cheek pouches induced by Salley way and obstructed by herba erigerontis were studied. Ink perfusion, analysis of images, resin cast form of micro-vessel, α-SMA detection and histopathological examination were applied to observe their changing patterns. Results　The rate of leukoplakia canceration of herba erigerontis group was one time lower than that of model group. The rate of normal cells was about four times more than that of model. α-SMA of herba erigerontis was higher than that of model (P<0.001). There was no significant difference between angeion vessel area and density (P>0.05). Nevertheless, the resin cast form of micro-vessel showed that its configuration and spacious arrangement was similar to control group. Conclusions　Herba erigerontis has the function of preventing leukoplakia canceration. It has no obvious effect on angio-hyperplasia and expansion in the course of leukoplakia canceration. However, it has the protecting function on angio-configuration and spacious arrangement and the complete of angeion vessel wall. Probably its mechanism is to promote blood circulation by removing blood stasis and benign angiopoiesis so as to resist canceration.