CAJ | 학술논문

目的探讨丹皮酚对过氧化氢(H2O2)诱导的PC12细胞凋亡的抑制作用及其机制.方法建立H2O2致PC12细胞损伤模型,采用MTT法测定细胞存活率,流式细胞术测定细胞凋亡率及细胞内活性氧含量,化学比色法测定乳酸脱氢酶(LDH)释放量及细胞内丙二醛(MDA)含量.结果 PC12细胞经H2O2 100 μmol·L-1处理10 h可致细胞存活率下降,并能诱导细胞凋亡,LDH释放量及细胞内活性氧和MDA含量明显增加;丹皮酚(12,25和50 μmol·L-1)预处理1 h可提高细胞存活率,减少细胞凋亡、LDH释放量及细胞内活性氧和MDA含量.结论丹皮酚对H2O2诱导的PC12细胞凋亡具有抑制作用,该作用可能与其抗氧化作用有关.
목적 탐토단피분대과양화경(H2O2)유도적PC12세포조망적억제작용급기궤제.방법 건립H2O2치PC12세포손상모형,채용MTT법측정세포존활솔,류식세포술측정세포조망솔급세포내활성양함량,화학비색법측정유산탈경매(LDH)석방량급세포내병이철(MDA)함량.결과 PC12세포경H2O2 100 μmol·L-1처리10 h가치세포존활솔하강,병능유도세포조망,LDH석방량급세포내활성양화MDA함량명현증가;단피분(12,25화50 μmol·L-1)예처리1 h가제고세포존활솔,감소세포조망、LDH석방량급세포내활성양화MDA함량.결론 단피분대H2O2유도적PC12세포조망구유억제작용,해작용가능여기항양화작용유관.
AIM To investigate the inhibitory effect of paeonol on hydrogen peroxide(H2O2)-induced apoptosis in PC12 cells. METHODS The injury model in PC12 cells was generated by H2O2 treatment. The cell viability was determined using methylthiazolyl tetrazolium reduction assay. Apoptotic cells and reactive oxygen species (ROS) were measured by flow cytometry. Lactate dehydrogenase (LDH) activity and malonyldialdehyde (MDA) content were measured by spectroscope respectively. RESULTS After PC12 cells were treated with H2O2 (100 μmol*L-1) for 10 h,its viability obviously decreased, and apoptotic cells, LDH release into the culture media, ROS and MDA contents in PC12 cells significantly increased. When the cells were pretreated with paeonol (12, 25 and 50 μmol*L-1)for 1 h prior to incubation with H2O2, its viability was greatly increased, and apoptotic cells, LDH release, ROS and MDA contents significantly decreased. CONCLUSION Paeonol protects PC12 cells from H2O2-induced apoptosis and this effect is probably achieved through its antioxidative action.